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Proteome profile of spleen in rock bream (Oplegnathus fasciatus) naturally infected with rock bream iridovirus (RBIV)
Genes & Genomics ( IF 2.1 ) Pub Date : 2021-08-24 , DOI: 10.1007/s13258-021-01149-1
Eun-Ji Ko 1 , Hyunsu Kim 1 , A-Reum Lee 1 , Kyung-Yoon Jeon 1 , Ahran Kim 2 , Do-Hyung Kim 3 , Chan-Il Park 4 , Yung Hyun Choi 5 , Suhkmann Kim 6 , Heui-Soo Kim 7 , Mee Sun Ock 1 , Hee-Jae Cha 1
Affiliation  

Background

Rock bream iridovirus (RBIV) is one of the most dangerous pathogens that causes the highest mortality in the aquaculture of rock bream (Oplegnathus fasciatus). Even though RBIV infection leads to huge economic loss, proteome studies on RBIV-infected rock bream have not been conducted to provide information about the differential protein expression pattern by the host protection system.

Objective

The purpose of this study was to investigate the protein expression patterns in spleens of rock bream olive after infection by RBIV or mixed infection by RBIV and bacteria.

Methods

Depending on the infection intensity and sampling time point, fish were divided into five groups: uninfected healthy fish at week 0 as the control (0C), heavily infected fish at week 0 (0H), heavily mixed RBIV and bacterial infected fish at week 0 (0MH), uninfected healthy fish at week 3 (3C), and lightly infected fish at week 3 (3L). Proteins were extracted from the spleens of infected rock bream. We used 2-DE analysis with LC–MS/MS to investigate proteome changes in infected rock bream.

Results

The results of the LC–MS/MS analyses showed different protein expression profiles after infection. Proteins related to oxygen transport and energy generation, such as hemoglobin, beta-globin, and ATP synthase, were mostly expressed in the infected spleen. Whereas proteins involved in structure and cell movement, such as tubulin, myosin, actin binding proteins, and intermediate filament proteins, were down-regulated in the infected spleens. The protein expression profiles between infection by RBIV and mixed infection by RBIV and bacteria showed similar patterns.

Conclusions

Our results indicated that infection by RBIV or mixed infection by RBIV and bacteria triggered energy generation and oxygen-transport, but cell migration and constructional changes in the spleen were extremely decreased.



中文翻译:

自然感染岩鲷虹彩病毒 (RBIV) 的岩鲷 (Oplegnathus fasciatus) 脾脏的蛋白质组学特征

背景

岩鲷虹彩病毒(RBIV)是导致岩鲷( Oplegnathus fasciatus)水产养殖中死亡率最高的最危险病原体之一。尽管 RBIV 感染导致巨大的经济损失,但尚未对 RBIV 感染的岩鲷进行蛋白质组学研究,以提供有关宿主保护系统差异蛋白质表达模式的信息。

客观的

本研究的目的是研究RBIV感染或RBIV与细菌混合感染后橄榄岩鲷脾脏中的蛋白质表达模式。

方法

根据感染强度和采样时间点,将鱼分为五组:第 0 周未感染的健康鱼作为对照 (0C)、第 0 周重度感染的鱼 (0H)、重度混合 RBIV 和第 0 周的细菌感染鱼(0MH),第 3 周未感染的健康鱼 (3C),第 3 周轻度感染的鱼 (3L)。从感染的鲷鱼的脾脏中提取蛋白质。我们使用 2-DE 分析和 LC-MS/MS 来研究受感染岩鲷的蛋白质组变化。

结果

LC-MS/MS 分析的结果显示感染后不同的蛋白质表达谱。与氧运输和能量产生相关的蛋白质,如血红蛋白、β-珠蛋白和 ATP 合酶,主要在受感染的脾脏中表达。而参与结构和细胞运动的蛋白质,如微管蛋白、肌球蛋白、肌动蛋白结合蛋白和中间丝蛋白,在受感染的脾脏中被下调。RBIV 感染与 RBIV 和细菌混合感染之间的蛋白质表达谱显示出相似的模式。

结论

我们的研究结果表明,RBIV 感染或 RBIV 和细菌的混合感染触发了能量产生和氧气运输,但脾脏中的细胞迁移和结构变化大大减少。

更新日期:2021-08-25
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