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Schirmer paper tear sampling of human eye diseases for paper spray mass spectrometry analysis
International Journal of Mass Spectrometry ( IF 1.8 ) Pub Date : 2021-08-24 , DOI: 10.1016/j.ijms.2021.116689
Dandan Di 1 , Shen-Hui Cai 1 , Weini Chen 1 , Zi-Cheng Yuan 1 , Zhaohan Wang 2 , Zhengxu Huang 1 , Bin Hu 1
Affiliation  

Paper spray mass spectrometry (PSMS) is a powerful analytical technique for direct analysis of human body fluids. Schirmer paper is commonly used to collect human tears in clinical examination. In this work, Schirmer paper was used for noninvasive sampling and storing of healthy and disease-infected human tears, and was then performed PSMS for direct tear analysis under both positive and negative detection modes. 24 healthy tear samples, 34 tear samples of meibomain gland dysfunction, 44 tear samples of senile cataract, and 32 tear samples of allergic conjunctivitis were investigated in this work. Screening potential biomarkers of different eye diseases were carried out by combining multivariate analysis methods. 41 positive ions and 53 negative ions were proposed to be potential biomarkers in this work. The capacity of Schirmer paper for storing tear sample at different temperatures was investigated. The present study shows that direct coupling Schirmer PSMS with multivariate analysis could be a simple, rapid, and in vivo method for screening the biomarkers from human tears that involved different eye diseases.



中文翻译:

用于纸喷雾质谱分析的人眼疾病的 Schirmer 纸泪液采样

纸喷雾质谱 (PSMS) 是一种用于直接分析人体体液的强大分析技术。Schirmer纸通常用于在临床检查中收集人类眼泪。在这项工作中,Schirmer 纸用于健康和受疾病感染的人类眼泪的无创采样和存储,然后在阳性和阴性检测模式下进行 PSMS 直接眼泪分析。24份健康泪液样本、34份睑板腺功能障碍泪液样本、44份老年性白内障泪液样本、32份过敏性结膜炎泪液样本。结合多元分析方法筛选不同眼病的潜在生物标志物。在这项工作中,41 个正离子和 53 个负离子被提议作为潜在的生物标志物。研究了 Schirmer 纸在不同温度下储存泪液样本的能力。目前的研究表明,直接耦合 Schirmer PSMS 与多变量分析可能是一种简单、快速和从涉及不同眼部疾病的人类眼泪中筛选生物标志物的体内方法。

更新日期:2021-09-03
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