The poor situational awareness about the spreading of the virus especially in the underdeveloped regions calls for novel virus assays of low cost and simple operation. Currently, such assays are exclusively restricted to nucleic acid detection. In this investigation, a virus protein serum assay has been proposed in a one-step and reagent-less route. Specifically, in this assay, the main protease of the virus is targeted by a short probe mimicking its substrate. While the probe-protein interaction brings them together, a fluorescent thiol targeting molecule reacts with the free thiol groups on the target protein near the probe, generating a fluorescence signal proportional to the concentration of the target. This induces an electroactive 2D peptide nano-network on the sensing surface only in the presence of the target protein. The sensitivity of the method is enhanced through potential electrochemical scanning during incubation with serum samples. The successful detection of the virus marker protein in the serum of the infected patients encourages further development of incorporation of this method into clinical practice.

对病毒传播的态势感知不足，尤其是在欠发达地区，需要低成本和操作简单的新型病毒检测方法。目前，此类测定仅限于核酸检测。在这项调查中，提出了一种病毒蛋白血清检测方法，采用一步法和无试剂途径。具体而言，在该测定中，病毒的主要蛋白酶被模拟其底物的短探针靶向。当探针-蛋白质相互作用将它们聚集在一起时，荧光硫醇靶向分子与探针附近目标蛋白质上的游离硫醇基团发生反应，产生与目标浓度成比例的荧光信号。只有在目标蛋白存在的情况下，这才会在传感表面诱导电活性 2D 肽纳米网络。该方法的灵敏度通过在与血清样品孵育期间的电化学扫描得到增强。在受感染患者的血清中成功检测到病毒标记蛋白，鼓励进一步开发将该方法纳入临床实践。