Some rare sugars can be potently medicinal, such as l-gulose. In this study, we present a novel alditol oxidase (fAldOx) from the soil fungus Penicillium sp. KU–1, and its application for the effective production of l-gulose. To the best of our knowledge, this is the first report of a successful direct conversion of d-sorbitol to l-gulose. We further purified it to homogeneity with a ∼108-fold purification and an overall yield of 3.26%, and also determined the effectors of fAldOx. The enzyme possessed broad substrate specificity for alditols such as erythritol (k_cat/K_M, 355 m⁻¹ s⁻¹), thus implying that the effective production of multiple rare sugars for medicinal applications may be possible.

一些稀有糖类具有很强的药用价值，例如l-古洛糖。在这项研究中，我们提出了一种来自土壤真菌Penicillium sp.的新型糖醇氧化酶 ( f AldOx) 。KU-1，及其在有效生产l-古洛糖中的应用。据我们所知，这是d-山梨糖醇成功直接转化为l-古洛糖的第一份报告。我们进一步将其纯化至均质，纯化率约为 108 倍，总产率为 3.26%，并确定了f AldOx的效应物。该酶对赤藓糖醇等糖醇具有广泛的底物特异性 ( k _cat / K _M , 355 m ^-1  s ^-1 )，因此意味着有效生产多种用于药用的稀有糖是可能的。