Abstract

Zinc deficiency in human body has gradually become a major challenge to public health in modern society. In our previous work, a novel polysaccharide from Inonotus obliquus IP3a was successfully isolated and purified. The preparation process of IP3a-Zn was optimized by response surface methodology based on single factor experiments. Characterization and antioxidant activities of IP3a-Zn were investigated. The optimal preparation process of IP3a-Zn was obtained as follows: chelation time was 6.3 h, zinc (II) initial concentration was 4.6 mg/mL and mass ratio of IP3a to zinc sulfate heptahydrate was 4.8:1(mg/mg). The weight loss of IP3a-Zn was slightly smaller than that of IP3a within the heating temperature range. After chelation with zinc, IP3a-Zn had crystalline properties like IP3a. IP3a-Zn had stronger antioxidant activity than IP3a. The results indicated that chelation with zinc enhanced the antioxidant activity of IP3a. IP3a-Zn may serve as a good source of zinc as a supplement and could be used as an antioxidant for the food industries.

摘要

人体缺锌已逐渐成为现代社会公共卫生的一大挑战。在我们之前的工作中，一种来自Innonotus Obliquus的新型多糖IP3a 被成功分离和纯化。基于单因素实验，采用响应面法优化了IP3a-Zn的制备工艺。研究了 IP3a-Zn 的表征和抗氧化活性。得到IP3a-Zn的最佳制备工艺如下：螯合时间为6.3 h，锌(II)初始浓度为4.6 mg/mL，IP3a与七水硫酸锌的质量比为4.8:1(mg/mg)。在加热温度范围内，IP3a-Zn 的失重略小于 IP3a。与锌螯合后，IP3a-Zn 具有与 IP3a 一样的结晶特性。IP3a-Zn 比 IP3a 具有更强的抗氧化活性。结果表明，与锌螯合增强了IP3a的抗氧化活性。