Peroxynitrite is an important chemical in the human immune system, which has high biocidal activity and can resist the invasion of pathogens. Currently, in vitro generation of peroxynitrite faces major technological challenges, especially for gas-phase production, which requires low-pressure conditions. Here, we report the method of how to generate gas-phase peroxynitrite at atmospheric pressure using dielectric barrier discharge. Results show that the peroxynitrite concentration positively correlates with energy density. Moreover, Penicillium digitatum, a common fungus contaminant greatly affecting food and fruit preservation, was used as a microbial pathogen model to confirm the biocidal effects of ozone-free nitrogen oxides under different discharge conditions. The germination inhibition efficiency of P. digitatum is highly likely attributed to the synergistic effect of gaseous peroxynitrite and NO.

中文翻译：

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在大气压下使用介质阻挡放电产生气相过氧亚硝酸盐：一种预期的灭菌器

过氧亚硝酸盐是人体免疫系统中的重要化学物质，具有较高的杀菌活性，能抵抗病原体的侵袭。目前，过氧亚硝酸盐的体外生成面临重大技术挑战，特别是对于需要低压条件的气相生产。在这里，我们报告了如何使用介质阻挡放电在大气压下生成气相过氧亚硝酸盐的方法。结果表明过氧亚硝酸盐浓度与能量密度呈正相关。此外，指状青霉（Penicillium digitatum）是一种严重影响食品和水果保存的常见真菌污染物，被用作微生物病原体模型，以确认不同排放条件下无臭氧氮氧化物的杀生物作用。萌发抑制效率P. digitatum很可能归因于气态过氧亚硝酸盐和 NO 的协同作用。