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Inhibition of Streptococcus mutans biofilms with bacterial-derived outer membrane vesicles
BMC Microbiology ( IF 4.2 ) Pub Date : 2021-08-24 , DOI: 10.1186/s12866-021-02296-x
Yihui Wang 1 , Joseph P Hoffmann 1 , Sarah M Baker 1 , Kerstin Höner Zu Bentrup 1 , William C Wimley 2 , Joseph A Fuselier 3 , Jacob P Bitoun 1 , Lisa A Morici 1
Affiliation  

Biofilms are microbial communities surrounded by a self-produced extracellular matrix which protects them from environmental stress. Bacteria within biofilms are 10- to 1000-fold more resistant to antibiotics, making it challenging but imperative to develop new therapeutics that can disperse biofilms and eradicate infection. Gram-negative bacteria produce outer membrane vesicles (OMV) that play critical roles in communication, genetic exchange, cargo delivery, and pathogenesis. We have previously shown that OMVs derived from Burkholderia thailandensis inhibit the growth of drug-sensitive and drug-resistant bacteria and fungi. Here, we examine the antibiofilm activity of Burkholderia thailandensis OMVs against the oral biofilm-forming pathogen Streptococcus mutans. We demonstrate that OMV treatment reduces biofilm biomass, biofilm integrity, and bacterial cell viability. Both heat-labile and heat-stable components, including 4-hydroxy-3-methyl-2-(2-non-enyl)-quinoline and long-chain rhamnolipid, contribute to the antibiofilm activity of OMVs. When OMVs are co-administered with gentamicin, the efficacy of the antibiotic against S. mutans biofilms is enhanced. These studies indicate that bacterial-derived OMVs are highly effective biological nanoparticles that can inhibit and potentially eradicate biofilms.

中文翻译:

用细菌衍生的外膜囊泡抑制变形链球菌生物膜

生物膜是被自我产生的细胞外基质包围的微生物群落,可以保护它们免受环境压力。生物膜内的细菌对抗生素的耐药性是原来的 10 到 1000 倍,这使得开发能够分散生物膜和根除感染的新疗法具有挑战性但势在必行。革兰氏阴性菌产生外膜囊泡 (OMV),在通讯、基因交换、货物运送和发病机制中起着关键作用。我们之前已经表明,源自泰国伯克霍尔德氏菌的 OMV 可抑制药物敏感和耐药细菌和真菌的生长。在这里,我们检查了 Burkholderia thailandensis OMV 对口腔生物膜形成病原体变形链球菌的抗生物膜活性。我们证明 OMV 处理降低了生物膜生物量、生物膜完整性、和细菌细胞活力。热不稳定和热稳定成分,包括 4-hydroxy-3-methyl-2-(2-non-enyl)-quinoline 和长链鼠李糖脂,都有助于 OMV 的抗生物膜活性。当 OMV 与庆大霉素共同给药时,抗生素对变形链球菌生物膜的功效得到增强。这些研究表明,细菌衍生的 OMV 是高效的生物纳米颗粒,可以抑制并可能根除生物膜。
更新日期:2021-08-24
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