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GRUSP, an Universal Stress Protein, Is Involved in Gibberellin-dependent Induction of Flowering in Arabidopsis thaliana
Doklady Biochemistry and Biophysics ( IF 0.8 ) Pub Date : 2021-08-23 , DOI: 10.1134/s1607672921040062
D S Gorshkova 1, 2 , I A Getman 1 , L I Sergeeva 1, 3 , Vl V Kuznetsov 1, 2 , E S Pojidaeva 1
Affiliation  

Abstract

The effect of T-DNA insertion in the 3'-UTR region of Arabidopsis thaliana At3g58450 gene encoding the Germination-Related Universal Stress Protein (GRUSP) was studied. It was found that under a long-day condition this mutation delays transition to flowering of grusp-115 transgenic line that due to a reduced content of endogenous bioactive gibberellins GA1 and GA3 in comparison to the wild-type plants (Col-0). Exogenous GA accelerated flowering of both lines but did not change the time of difference in the onset of flowering between Col-0 and grusp-115. In addition to changes in GA metabolism, grusp-115 evidently has disturbances in realization of the signal that induces flowering. This is confirmed by the results of gene expression of the floral integrator FLOWERING LOCUS T (FT) and the floral repressor FLOWERING LOCUS C (FLC), which are key flowering regulators and acting opposite. We hypothesize that the formation of grusp-115 phenotype can also be affected by a low expression level of FT due to up-regulated FLC expression.



中文翻译:

GRUSP 是一种通用应激蛋白,参与赤霉素依赖性的拟南芥开花诱导

摘要

研究了T-DNA插入拟南芥At3g58450基因3'-UTR区的影响,该基因编码与萌发相关的通用应激蛋白(GRUSP)。发现在长日照条件下,这种突变延迟了grusp-115转基因品系向开花的转变,这是由于与野生型植物 (Col-0) 相比,内源性生物活性赤霉素 GA1 和 GA3 的含量降低。外源 GA 加速了两个品系的开花,但没有改变 Col-0 和grusp-115之间开花开始的时间差异。除了 GA 代谢的变化,grusp-115显然在实现诱导开花的信号方面存在干扰。花整合子FLOWERING LOCUS T ( FT ) 和花抑制子FLOWERING LOCUS C ( FLC )的基因表达结果证实了这一点,它们是关键的开花调节剂,作用相反。我们假设由于上调FLC表达, grusp-115表型的形成也可能受到FT低表达水平的影响。

更新日期:2021-08-24
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