Abstract

A reusable system for phenol determination in an aqueous medium was obtained by adsorption of extracellular oxidase from fungus Neonothopanus nambi onto modified nanodiamonds (MND) synthesized by detonation. It was found that the enzyme strongly binds to MND and exhibits catalytic activity in the reaction of co-oxidation of phenol with 4-aminoantipyrine without the addition of hydrogen peroxide. In the presence of the MND–oxidase complex, a significantly (by an order of magnitude) higher yield of the reaction product is recorded as compared to the yield in the presence of a free enzyme; the mechanism of the revealed effect is discussed. Model experiments have demonstrated the multiple use of the MND–oxidase complex for testing phenol in aqueous samples. The immobilized enzyme exhibits functional activity during long-term (2 months) storage of the MND–oxidase complex at 4°C. The data obtained create the prerequisites for using the created system in environmental monitoring of water pollution with phenol.

中文翻译：

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基于纳米金刚石和来自担子菌 Neonothopanus nambi 的细胞外氧化酶的水性介质中苯酚检测的可重复使用系统

摘要

通过吸附真菌Neonothopanus nambi的细胞外氧化酶获得了一种可重复使用的测定水介质中苯酚的系统到通过爆炸合成的改性纳米金刚石 (MND) 上。发现该酶与 MND 强烈结合，并在不添加过氧化氢的情况下，在苯酚与 4-氨基安替比林的共氧化反应中表现出催化活性。在存在 MND-氧化酶复合物的情况下，与存在游离酶时的产率相比，反应产物的产率显着（一个数量级）更高；讨论了揭示效应的机制。模型实验已证明 MND-氧化酶复合物可多次用于测试水性样品中的苯酚。固定化酶在 MND-氧化酶复合物在 4°C 下长期（2 个月）储存期间表现出功能活性。