Abstract

The present study investigated Boerhaavia diffusa extract against Mycobacterium tuberculosis H37Rv (M.tb) infection in vitro and explored the underlying mechanism. The study demonstrated that Boerhaavia diffusa extract significantly (p < 0.05) reduced RAW 264.7 and A549 cell viability in concentration dependent manner. In BEAS-2B, NuLi-1 cells and splenocytes no significant (p > 0.05) reduction in viability was observed on treatment with 2.5 to 20 mg/L concentrations of Boerhaavia diffusa. The M. tb‑induced increase in TNF‑α expression was significantly (p < 0.05) reversed by Boerhaavia diffusa treatment in RAW 264.7 and BEAS-2B cells. Moreover, Boerhaavia diffusa treatment significantly (p < 0.05) inhibited M.tb‑induced increase in IL-6 and IL‑1β expression in RAW 264.7 and BEAS-2B cells. Boerhaavia diffusa treatment of RAW 264.7 and BEAS-2B cells significantly (p < 0.05) reversed M.tb‑induced increase in iNOS and COX‑2 expression. Additionally, in Boerhaavia diffusa treated cells M.tb‑induced increase in NO release was significantly (p < 0.05) reduced compared to untreated cells. In summary, Boerhaavia diffusa treatment inhibits pro-inflammatory cytokine production, NO release and regulate immunomodulatory mediators in M.tb‑infected RAW 264.7 and BEAS-2B cells. Therefore, Boerhaavia diffusa may be developed as a therapeutic agent for treatment of M.tb‑infection.

中文翻译：

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Boerhaavia diffusa 提取物在体外作为特异性抗结核剂对抗结核分枝杆菌 H37Rv 感染

摘要

本研究调查了Boerhaavia diffusa提取物对体外结核分枝杆菌H37Rv ( M.tb ) 感染的影响，并探讨了其潜在机制。该研究表明，白花蛇舌草提取物以浓度依赖性方式显着降低了 RAW 264.7 和 A549 细胞活力（p < 0.05）。在 BEAS-2B 中，NuLi-1 细胞和脾细胞在用 2.5 至 20 mg/L 浓度的Boerhaavia diffusa处理时未观察到活力显着降低（p > 0.05）。在结核分枝杆菌中诱导增加的TNF- α表达显著（p通过反相<0.05）Boerhaavia diffusa在 RAW 264.7 和 BEAS-2B 细胞中的处理。此外，Boerhaavia diffusa处理显着（p < 0.05）抑制了M.tb诱导的 RAW 264.7 和 BEAS-2B 细胞中 IL-6 和 IL-1β 表达的增加。Boerhaavia diffusa对 RAW 264.7 和 BEAS-2B 细胞的处理显着（p < 0.05）逆转了M.tb诱导的 iNOS 和 COX-2 表达的增加。此外，在Boerhaavia diffusa处理的细胞中，与未处理的细胞相比，M.tb诱导的 NO 释放增加显着降低（p < 0.05）。综上所述，白花蛇舌草在M.tb感染的 RAW 264.7 和 BEAS-2B 细胞中，治疗抑制促炎细胞因子的产生、NO 释放并调节免疫调节介质。因此，Boerhaavia diffusa可能被开发为治疗M.tb感染的治疗剂。