Stroke remains a significant unmet need in the clinic with few therapeutic options. We, and others, have implicated the role of inflammatory microbiota in stroke secondary cell death. Elucidating this inflammation microbiome as a biomarker may improve stroke diagnosis and treatment. Here, adult Sprague-Dawley rats performed 30 minutes of exercise on a motorized treadmill for 3 consecutive days prior to transient middle cerebral artery occlusion (MCAO). Stroke animals that underwent exercise showed 1) robust behavioral improvements, 2) significantly smaller infarct sizes and increased peri-infarct cell survival and 3) decreasing trends of inflammatory microbiota BAC303, EREC482, and LAB158 coupled with significantly reduced levels of inflammatory markers ionized calcium binding adaptor molecule 1, tumor necrosis factor alpha, and mouse monoclonal MHC Class II RT1B in the brain, gut, spleen, and thymus compared to non-exercised stroke rats. These results suggest that a specific set of inflammatory microbiota exists in central and peripheral organs and can serve as a disease biomarker and a therapeutic target for stroke.

中风在临床上仍然是一个重要的未满足需求，几乎没有治疗选择。我们和其他人已经暗示炎症微生物群在中风继发性细胞死亡中的作用。阐明这种炎症微生物组作为生物标志物可能会改善中风的诊断和治疗。在这里，成年 Sprague-Dawley 大鼠在短暂的大脑中动脉闭塞 (MCAO) 之前连续 3 天在电动跑步机上进行 30 分钟的运动。接受运动的中风动物表现出 1) 显着的行为改善，2) 梗死面积显着缩小，梗死周围细胞存活率增加，3) 炎症微生物群 BAC303、EREC482 和 LAB158 呈下降趋势，同时炎症标志物离子钙结合水平显着降低衔接分子1，肿瘤坏死因子α，与未运动的中风大鼠相比，大脑、肠道、脾脏和胸腺中的小鼠单克隆 MHC II 类 RT1B。这些结果表明，一组特定的炎症微生物群存在于中枢和外周器官中，可以作为疾病生物标志物和中风的治疗靶点。