Mineral coatings manipulate surface properties such as roughness, porosity, wettability and surface energy. Properties that are known to determine cell behaviour. Therefore, mineral coatings can potentially be used to manipulate cell fate. This paper studies mineral-cell interactions through coatings in a stacked cell culture platform. Minerals were chosen according to their influence on Human Dermal Fibroblasts (HDFs): calcium carbonate, calcium sulphates, and kaolin. Mineral coatings were formulated with the additives latex, sorbitol, polyvinyl alcohol (PVOH) and TEMPO-oxidised cellulose nanofibrils (CNF-T). The coatings were placed as a bottom or top of the device, for a direct or indirect interaction with HDFs, respectively. Cells were seeded, in various densities, to the bottom of the device; and cell density and confluency were monitored in time. Overall, results show that the coating interaction is influenced at first by the cell seeding density. Scarce cell seeding density limits adaptability to the new environment, while an abundant one encourages confluency in time. In between those densities, coating formulation plays the next major role. Calcium carbonate promoted HDFs growth the most as expected, but the response to the rest of minerals depended on the coating additive. CNF-T encouraged proliferation even for kaolin, a mineral with long-term toxicity to HDFs, while PVOH induced a detrimental effect on HDF growth regardless of the mineral. At last, the placement of the coated layer provided insights on the contact-dependency of each response. This study highlights the importance of the experimental design, including coating formulation, when investigating cellular response to biomaterials.

矿物涂料控制表面特性，例如粗糙度、孔隙率、润湿性和表面能。确定细胞行为的已知特性。因此，矿物涂层可潜在地用于操纵细胞命运。本文通过堆叠细胞培养平台中的涂层研究矿物质与细胞的相互作用。根据对人真皮成纤维细胞 (HDF) 的影响选择矿物质：碳酸钙、硫酸钙和高岭土。矿物涂料由乳胶、山梨糖醇、聚乙烯醇 (PVOH) 和 TEMPO 氧化纤维素纳米原纤维 (CNF-T) 等添加剂配制而成。涂层被放置在设备的底部或顶部，分别用于与 HDF 的直接或间接相互作用。将不同密度的细胞接种到装置底部；并及时监测细胞密度和汇合度。总体而言，结果表明涂层相互作用首先受细胞接种密度的影响。稀少的细胞接种密度限制了对新环境的适应性，而丰富的细胞接种密度会促进及时融合。在这些密度之间，涂料配方起着下一个主要作用。正如预期的那样，碳酸钙最能促进 HDF 的生长，但对其余矿物质的反应取决于涂料添加剂。CNF-T 甚至促进高岭土的增殖，高岭土是一种对 HDF 具有长期毒性的矿物质，而 PVOH 对 HDF 的生长产生不利影响，而不管矿物质如何。最后，涂层的放置提供了对每个响应的接触依赖性的见解。这项研究强调了实验设计的重要性，