In recent years, more attention has been paid to the application of cold plasma (CP) in eliminating foodborne pathogenic bacteria. This work investigated CP effects on inactivation kinetics and cell envelopes of Listeria monocytogenes (L. monocytogenes) and Salmonella Enteritidis (S. Enteritidis). Bacterial suspensions were treated with dielectric barrier discharge atmospheric CP at 75 kV for different treatment time. Three regression models were tested for estimating inactivation kinetics. Reactive species generated in plasma, the appearance and integrity of bacterial cells, the activity and secondary structure of enzymes in the cell envelope, and molecular docking, were measured for evaluating the envelope damages. Results indicated that Log-linear model was suitable for L. monocytogenes and the Weibull model was suitable for S. Enteritidis. S. Enteritidis was more sensitive to short-lived reactive species (such as OH radicals) in plasma than L. monocytogenes, and the cell envelope of S. Enteritidis was more severely damaged (the increased membrane permeability and leakage of intracellular substances) after plasma treatment. Interestingly, compared with S. Enteritidis, the decrease in the activity of enzymes existing in the cell envelope of L. monocytogenes did not contribute significantly to the death of bacteria. Molecular docking further suggested that the decrease in the enzyme activity might be due to the modification of the enzyme, by the interaction between reactive species in plasma (H₂O₂) and amino acid residues of the enzyme through the hydrogen bond.

近年来，冷等离子体（CP）在消除食源性病原菌中的应用受到越来越多的关注。这项工作研究了 CP 对单核细胞增生李斯特菌( L. monocytogenes ) 和肠炎沙门氏菌 ( S.肠炎）。细菌悬浮液在 75 kV 下用介质阻挡放电大气 CP 处理不同的处理时间。测试了三个回归模型以估计失活动力学。测量血浆中产生的活性物质、细菌细胞的外观和完整性、细胞包膜中酶的活性和二级结构以及分子对接，以评估包膜损伤。结果表明，对数线性模型是适用于L. monocytogene S和维泊尔模型是适合S.肠炎。S.肠炎是在血浆比短命反应性物质（例如OH基）更敏感单增李斯特菌，以及细胞被膜S.等离子处理后肠炎的损伤更为严重（膜通透性增加和细胞内物质渗漏）。有趣的是，相比S.肠炎，在现有的细胞膜酶的活性降低单增李斯特菌没有显著细菌的死亡作出贡献。分子对接进一步表明，酶活性的降低可能是由于酶的修饰，血浆中的活性物质 (H ₂ O ₂ ) 与酶的氨基酸残基之间通过氢键相互作用。