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The effects of quercetin combined with nucleopolyhedrovirus on the growth and immune response in the silkworm (Bombyx mori)
Archives of Insect Biochemistry and Physiology ( IF 2.2 ) Pub Date : 2021-08-24 , DOI: 10.1002/arch.21839
Guiqin Shi 1 , Zhaoyang Kang 1 , Huijuan Liu 1 , Fei Ren 1 , Yuan Zhou 1
Affiliation  

Flavonoids are secondary metabolites that help plants resist insect attack. It can resist insect attack by inhibiting insect immune defense, and pathogens can also inhibit insect immune defense. It is speculated that the combination of flavonoids and pathogens may inhibit the immune defense and have stronger toxicity to silkworm. In this study, the combined treatment of quercetin with Bombyx mori nuclear polyhedrosis virus (BmNPV) had significant negative effects on the growth and survival of silkworm compared with BmNPV group. The detoxifying enzyme activity of BmNPV group was significantly increased at 96 h, while the activity of the combined treatment group was significantly decreased with the increase of quercetin exposure time (72 or 96 h). The activity of antioxidant enzymes also showed a similar trend, that was, the activity of antioxidant enzymes in the combined treatment group also decreased significantly with the increase of quercetin exposure time, which led to the increase of reactive oxygen species content. The silkworm cells would produce lipid peroxidation, malondialdehyde content was significantly increased, so that the expression of immune-related genes (the antimicrobial peptide, Toll pathway, IMD pathway, JAK-STAT pathway, and melanin genes) were decreased, leading to the damage of the immune system of silkworm. These results indicated that quercetin combined with BmNPV could inhibit the activities of protective enzymes and lead to oxidative damage to silkworm. It can also affect the immune response of the silkworm, and thus resulting in abnormal growth. This study provides the novel conclusion that quercetin accumulation will increase the susceptibility of silkworm to pathogens.

中文翻译:

槲皮素联合核多角体病毒对家蚕(Bombyx mori)生长和免疫反应的影响

类黄酮是帮助植物抵抗昆虫侵袭的次生代谢物。它可以通过抑制昆虫免疫防御来抵抗昆虫的攻击,病原体也可以抑制昆虫免疫防御。推测黄酮类化合物与病原体的结合可能抑制免疫防御,对家蚕具有更强的毒性。本研究将槲皮素与家蚕联合治疗与BmNPV组相比,核多角体病毒(BmNPV)对家蚕的生长和存活有显着的负面影响。BmNPV组的解毒酶活性在96 h时显着升高,而联合治疗组的活性随着槲皮素暴露时间(72或96 h)的增加而显着降低。抗氧化酶的活性也表现出类似的趋势,即联合处理组抗氧化酶的活性也随着槲皮素暴露时间的增加而显着降低,从而导致活性氧含量增加。家蚕细胞会产生脂质过氧化,丙二醛含量显着增加,使免疫相关基因(抗菌肽、Toll通路、IMD通路、JAK-STAT 通路和黑色素基因)减少,导致家蚕免疫系统受损。这些结果表明槲皮素联合BmNPV可抑制保护酶的活性,导致家蚕氧化损伤。它还可以影响家蚕的免疫反应,从而导致异常生长。该研究提供了新的结论,即槲皮素的积累会增加家蚕对病原体的易感性。
更新日期:2021-09-20
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