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Erylysin A inhibits cytokinesis in Escherichia coli by binding with cardiolipin
The Journal of Biochemistry ( IF 2.7 ) Pub Date : 2021-04-23 , DOI: 10.1093/jb/mvab052
Tomoko Sakihara 1 , Naoko Takiguchi 1 , Hikari Uzawa 1 , Rika Serizawa 1 , Tetsuyuki Kobayashi
Affiliation  

Cardiolipin (CL) localizes to curved membranes such as cristae in mitochondria as well as cell poles and division sites in rod-shaped bacteria. CL is believed to stabilize the membrane curvature by localizing to sites of negative curvature. However, this hypothesis has not been tested because of a lack of appropriate tools to distinguish CL inside and outside lipid bilayers. In this study, we provided the first evidence that CL localized to regions of negative curvature in Escherichia coli using the novel CL probe erylysin A-EGFP (EryA-EGFP). Staining in E.coli illustrated that CL localized to the inner leaflets at cell poles and the outer leaflets at division sites. Furthermore, we revealed that EryA-EGFP inhibited cytokinesis. We propose that cytokinesis completes after CL in the outer leaflets transfers to the inner leaflets at division sites by inspecting the mechanism of inhibition of cytokinesis. Moreover, the cytoskeletal protein RodZ was abnormally distributed when cytokinesis was inhibited by EryA-EGFP, suggesting that RodZ participates in cytokinesis. In summary, we revealed the detailed distribution of CL and proposed a new model of cytokinesis.

中文翻译:

Erylysin A 通过与心磷脂结合抑制大肠杆菌胞质分裂

心磷脂 (CL) 定位于弯曲的膜,例如线粒体中的嵴以及杆状细菌中的细胞极和分裂位点。CL 被认为通过定位到负曲率位点来稳定膜曲率。然而,由于缺乏适当的工具来区分脂质双层内外的 CL,这一假设尚未得到验证。在这项研究中,我们提供了第一个证据,证明 CL 使用新型 CL 探针 erylysin A-EGFP (EryA-EGFP) 定位于大肠杆菌中的负曲率区域。大肠杆菌中的染色表明 CL 定位于细胞极的内叶和分裂部位的外叶。此外,我们发现 EryA-EGFP 抑制胞质分裂。我们通过检查胞质分裂的抑制机制,提出胞质分裂在外叶中的 CL 转移到分裂部位的内叶后完成。此外,细胞骨架蛋白RodZ在EryA-EGFP抑制胞质分裂时分布异常,表明RodZ参与胞质分裂。总之,我们揭示了 CL 的详细分布,并提出了一种新的胞质分裂模型。
更新日期:2021-04-23
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