Bacteriocins produced by lactic acid bacteria have potential use as natural food preservatives, which may alleviate current problems associated with the overuse of antibiotics and emerging multi-drug-resistant microbes. In this work, Lactiplantibacillus plantarum RUB1 was found to produce a class IIb bacteriocin with strong antibacterial activity. Except for plnXY encoding putative proteins, L. plantarum RUB1 contains most genes in five operons (plnABCD, plnGHSTUVW, plnMNOP, plnIEF, and plnRLJK) related to bacteriocin synthesis. Adding low (100 and 500 ng/mL) and medium (1 μg/mL) concentrations of PlnA to broth promoted bacteriocin production and upregulated bacteriocin gene plnA, while high concentrations (50 and 200 μg/mL) inhibited expression of these genes. Co-culturing L. plantarum RUB1 with Enterococcus hirae 1003, Enterococcus hirae LWS, Limosilactobacillus fermentum RC4, L. plantarum B6, and even Listeria monocytogenes ATCC 19111 and Staphylococcus aureus ATCC 6538 enhanced bacteriocin activity and expression of bacteriocin-related genes. This study verifies that PlnA can indeed upregulate the expression of bacteriocin genes, and also bacteriocin production can be induced by co-culture with some specific bacteria or their cell-free supernatants. Bacteriocin production by L. plantarum RUB1 is mediated by a quorum sensing mechanism, directly influenced by autoinducing peptide or specific strains. The findings provide new methods and insight into bacteriocin production mechanisms.

乳酸菌产生的细菌素具有作为天然食品防腐剂的潜在用途，可以缓解当前与抗生素过度使用和新出现的多重耐药微生物相关的问题。在这项工作中，发现植物乳杆菌RUB1 可产生具有强抗菌活性的 IIb 类细菌素。除了pln XY 编码推定的蛋白质外，植物乳杆菌RUB1 包含五个操纵子中的大多数基因（pln ABCD、pln GHSTUVW、pln MNOP、pln IEF 和plnRLJK) 与细菌素合成有关。在肉汤中添加低（100 和 500 ng/mL）和中等（1 μg/mL）浓度的 PlnA 促进了细菌素的产生并上调了细菌素基因pln A，而高浓度（50 和 200 μg/mL）抑制了这些基因的表达。植物乳杆菌RUB1 与Enterococcus hirae 1003、Enterococcus hirae LWS、发酵乳杆菌RC4、植物乳杆菌B6，甚至单核细胞增生李斯特菌ATCC 19111 和金黄色葡萄球菌共培养ATCC 6538 增强了细菌素活性和细菌素相关基因的表达。本研究证实PlnA确实可以上调细菌素基因的表达，并且与某些特定细菌或其无细胞上清液共培养可以诱导细菌素的产生。植物乳杆菌RUB1产生的细菌素由群体感应机制介导，直接受自诱导肽或特定菌株的影响。这些发现为细菌素生产机制提供了新的方法和见解。