ABSTRACT

Background

Salivary mucin MUC5B seems to promote biodiversity in dental biofilms, and thereby oral health, for example, by inducing synergistic ‘mucolytic’ activities in a variety of microbial species that need to cooperate for the release of nutrients from the complex glycoprotein. Knowledge of how early colonizers interact with host salivary proteins is integral to better understand the maturation of putatively harmful oral biofilms and could provide key insights into biofilm physiology.

Methods

The early oral colonizer Streptococcus gordonii DL1 was grown planktonically and in biofilm flow cell systems with uncoated, MUC5B or low-density salivary protein (LDP) coated surfaces. Bacterial cell proteins were extracted and analyzed using a quantitative mass spectrometry-based workflow, and differentially expressed proteins were identified.

Results and conclusions

Overall, the proteomic profiles of S. gordonii DL1 were similar across conditions. Six novel biofilm cell proteins and three planktonic proteins absent in all biofilm cultures were identified. These differences may provide insights into mechanisms for adaptation to biofilm growth in this species. Salivary MUC5B also elicited specific responses in the biofilm cell proteome. These regulations may represent mechanisms by which this mucin could promote colonization of the commensal S. gordonii in oral biofilms.

中文翻译：

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戈登链球菌 DL1 生物膜细胞在唾液 MUC5B 附着过程中的蛋白质组学反应

摘要

背景

唾液粘蛋白​​ MUC5B 似乎可以促进牙齿生物膜中的生物多样性，从而促进口腔健康，例如，通过在需要合作从复杂糖蛋白中释放营养的多种微生物物种中诱导协同“粘液溶解”活性。了解早期殖民者如何与宿主唾液蛋白相互作用对于更好地了解假定有害的口腔生物膜的成熟是不可或缺的，并且可以提供对生物膜生理学的重要见解。

方法

早期的口腔定植菌 Streptococcus gordonii DL1 在浮游生物膜流通池系统中生长，表面未包被 MUC5B 或低密度唾液蛋白 (LDP) 包被。使用基于定量质谱的工作流程提取和分析细菌细胞蛋白质，并鉴定差异表达的蛋白质。

结果和结论

总体而言， S. gordonii DL1的蛋白质组学特征在不同条件下相似。鉴定了所有生物膜培养物中不存在的六种新型生物膜细胞蛋白和三种浮游蛋白。这些差异可能为该物种适应生物膜生长的机制提供见解。唾液 MUC5B 也在生物膜细胞蛋白质组中引起特异性反应。这些规定可能代表了这种粘蛋白可以促进共生S. gordonii在口腔生物膜中定植的机制。