We studied the lamellar level correlations between the local composition and local mechanical properties in the femur of two inbred strains of mice (A/J and C57BL/6J (B6)), with known differences in the average mineralization and long-bone mechanical properties, to gain insights into how their extracellular matrix is mineralized. The local elastic moduli and indentation yield strengths were determined using spherical nanoindentation stress-strain analysis, while Raman spectroscopy was used to determine the local composition around the indents in a total of 11 samples. Our results show a significant difference in the mineral-to-matrix ratio of the two strains of mice, with the A/J mice showing an overall higher mineral-to-matrix ratio and lower carbonate substitution in the mineral. These differences are prominent in the newer bone and become less significant as the bone matures. Additionally, local mineral-to-matrix ratio was found to be a good indicator of the local mechanical properties.

我们研究了两种近交系小鼠（A/J 和 C57BL/6J (B6)）股骨局部组成和局部力学性能之间的层状水平相关性，已知平均矿化和长骨力学性能存在差异，深入了解它们的细胞外基质是如何矿化的。使用球形纳米压痕应力应变分析确定局部弹性模量和压痕屈服强度，而拉曼光谱用于确定总共 11 个样品中压痕周围的局部成分。我们的结果表明，两种小鼠品系的矿物质与基质的比例存在显着差异，A/J 小鼠的矿物质与基质的比例总体较高，矿物质中的碳酸盐取代度较低。这些差异在较新的骨骼中很突出，随着骨骼的成熟而变得不那么明显。此外，发现局部矿物与基质的比率是局部机械性能的良好指标。