Background

: Mogroside V, the main ingredient of Siraitia grosvenorii, has been proved to have therapeutic effects on pulmonary diseases. The specific mechanism still remains to be clarified, which hinders the potence of its medicinal value.

Purpose

: Serum and lung metabolomics based on LC-MS analysis were applied to explore the mechanism of mogroside V against lung inflammation.

Method

: In this study, balb/c mice were divided into control, model, mogeoside V and SH groups. We evaluated the protective effects of mogroside V on lung inflammation in asthmatic mice. Suhuang Zhike Jiaonang was used as positive drug. Metabolic profiles of serum and lung samples of mice in control, model and mogroside V groups were analyzed by LC-MS.

Results

: Administration of mogroside V effectively relieved the expression of biochemical cytokines and lung inflammatory infiltration of asthmatic mice caused by ovalbumin (OVA). And visceral index of mice treated with mogroside V was close to control group. These results indicated that mogroside V ameliorated OVA-induced lung inflammation. LC-MS based metabolomics analysis demonstrated 6 main pathways in asthmatic mice including Vitamin B6 metabolism, Taurine and hypotaurine metabolism, Ascorbate and aldarate metabolism, Histidine metabolism, Pentose and glucuronate interconversions, Citrate cycle (TCA cycle) were regulated after using mogroside V.

Conclusion

: The study firstly elucidates the metabolic pathways regulated by mogroside V on lung inflammation through metabolomics, providing a theoretical basis for more sufficient utilization and compatibility of mogroside V.

中文翻译：

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基于肺和血清代谢组学的罗汉果苷 V 减轻 OVA 诱导的肺部炎症

背景

：罗汉果的主要成分罗汉果苷 V已被证明对肺部疾病有治疗作用。具体机制仍有待阐明，这阻碍了其药用价值的发挥。

目的

：应用基于LC-MS分析的血清和肺代谢组学来探索罗汉果苷V抗肺部炎症的机制。

方法

：在这项研究中，balb/c 小鼠被分为对照、模型、mogeoside V 和 SH 组。我们评估了罗汉果苷 V 对哮喘小鼠肺部炎症的保护作用。苏黄止咳胶囊作为阳性药。通过LC-MS分析对照组、模型组和罗汉果苷V组小鼠血清和肺样品的代谢谱。

结果

：罗汉果苷 V 的给药有效缓解了卵清蛋白 (OVA) 引起的哮喘小鼠生化细胞因子的表达和肺部炎症浸润。罗汉果苷V治疗组小鼠内脏指数接近对照组。这些结果表明罗汉果苷 V 改善了 OVA 诱导的肺部炎症。基于 LC-MS 的代谢组学分析表明，哮喘小鼠的 6 条主要途径包括维生素 B6 代谢、牛磺酸和亚牛磺酸代谢、抗坏血酸和醛糖酸盐代谢、组氨酸代谢、戊糖和葡萄糖醛酸相互转化、使用罗汉果苷 V 后调节柠檬酸循环（TCA 循环）。

结论

：该研究首次通过代谢组学阐明罗汉果苷V对肺部炎症的调控代谢途径，为罗汉果苷V更充分的利用和配伍提供理论依据。