This study aimed to determine the lysine requirement in tambatinga fingerlings (Colossoma macropomum × Piaractus brachypomum) using different diet formulation techniques. A total of 1,800 fingerlings (0.82 ± 0.39 g) were fed for 52 days with six isoenergetic diets (3,000 kcal energy kg⁻¹) containing different levels of lysine (12.9, 14.8, 16.6, 18.5, 20.4 and 22.2 g kg⁻¹) were formulated using amino acid supplementation and diet dilution techniques. There was a linear effect on lysine intake and a quadratic effect for weight gain, specific growth rate, feed conversion ratio, lysine efficiency for weight gain and fish body protein deposition up to estimated levels of 19.15; 19.43; 19.28; 15.92 and 19.27 g kg⁻¹, respectively. Fish-fed diets formulated by the dilution technique exhibited higher performance and body protein deposition. Body fat deposition and nitrogen retention efficiency of fish-fed diets formulated by the supplementation technique increased up to estimated levels of 17.96 and 19.06 g kg⁻¹, respectively. The recommended lysine level in diets for tambatinga fingerlings is 19.27 g kg⁻¹, for providing greater performance and body protein deposition of fishes, and dilution technique can be used in experimental fish diets formulation.

中文翻译：

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使用不同日粮配方技术对 tambatinga (♀Colossoma macropomum × ♂ Piaractus brachypomus) 鱼种的赖氨酸需求

本研究旨在使用不同的日粮配方技术确定 tambatinga 鱼种 ( Colossoma macropomum × Piaractus brachypomum ) 的赖氨酸需求。总共 1,800 条鱼种 (0.82 ± 0.39 g) 用六种含有不同水平赖氨酸（12.9、14.8、16.6、18.5、20.4 和 22.2 g kg ^-1）的等能量日粮（3,000 kcal 能量 kg ^-1 ）喂养了 52 天使用氨基酸补充和饮食稀释技术配制。赖氨酸摄入量呈线性效应，增重、比生长率、饲料转化率、赖氨酸增重效率和鱼体蛋白质沉积呈二次效应，估计水平高达 19.15；19.43；19.28；15.92 和 19.27 g kg ^-1， 分别。通过稀释技术配制的鱼食日粮表现出更高的性能和身体蛋白质沉积。通过补充技术配制的鱼饲日粮的体脂沉积和氮保留效率分别增加至估计的 17.96 和 19.06 g kg ^-1水平。推荐的 tambatinga 鱼种日粮中赖氨酸水平为 19.27 g kg ^-1，以提供更好的性能和鱼体蛋白质沉积，稀释技术可用于实验鱼日粮配方。