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PqsR-independent quorum-sensing response of Pseudomonas aeruginosa ATCC 9027 outlier-strain reveals new insights on the PqsE effect on RhlR activity
Molecular Microbiology ( IF 3.6 ) Pub Date : 2021-08-21 , DOI: 10.1111/mmi.14797
Selene García-Reyes 1 , Miguel Cocotl-Yañez 2 , Martín Paolo Soto-Aceves 1 , Abigail González-Valdez 1 , Luis Servín-González 1 , Gloria Soberón-Chávez 1
Affiliation  

Pseudomonas aeruginosa is a ubiquitous environmental bacterium and an opportunistic pathogen that represents an important health hazard. The quorum-sensing response regulates the expression of several virulence factors and involves three regulons: Las, Rhl, and Pqs. The P. aeruginosa ATCC 9027 strain, which belongs to the genetically diverse PA7 clade, contains a frame-shift mutation in the pqsR gene that encodes a transcriptional activator necessary for pyocyanin (PYO) synthesis in type strains PAO1 and PA14. Here we characterize the PqsE-dependent production of PYO in strain ATCC 9027. We show that this strain expresses pqsE independently of PqsR and in the absence of quinolone production, and that PqsE promotes the RhlR-dependent production of PYO, yet this production is not strictly dependent on PqsE. In addition, we show that in both strains ATCC 9027 and PAO1, PqsE overexpression causes an increased concentration of RhlR and enhances PYO production but does not affect rhamnolipids (RL) production in the same way. These results suggest that PqsE interaction with RhlR preferentially modifies its ability to activate transcription of genes involved in PYO production and provide new evidence about PqsE-dependent RhlR activation, highlighting the variability of the QS response among different P. aeruginosa clades and strains.

中文翻译:

铜绿假单胞菌 ATCC 9027 异常菌株的 PqsR 独立群体感应反应揭示了 PqsE 对 RhlR 活性影响的新见解

铜绿假单胞菌是一种普遍存在的环境细菌,是一种机会性病原体,是一种重要的健康危害。群体感应反应调节几种毒力因子的表达,并涉及三个调节子:Las、Rhl 和 Pqs。铜绿假单胞菌ATCC 9027 菌株属于遗传多样的 PA7 进化枝,在 pqsR 基因中包含移码突变,该基因编码类型菌株 PAO1 和 PA14 中绿脓素 (PYO) 合成所必需的转录激活因子。在这里,我们描述了菌株 ATCC 9027 中 PqsE 依赖的 PYO 产生。我们表明该菌株表达pqsE独立于 PqsR 并且在没有喹诺酮产生的情况下,并且 PqsE 促进了 RhlR 依赖性 PYO 的产生,但这种产生并不严格依赖于 PqsE。此外,我们发现在 ATCC 9027 和 PAO1 菌株中,PqsE 过表达会导致 RhlR 浓度增加并增强 PYO 的产生,但不会以相同的方式影响鼠李糖脂 (RL) 的产生。这些结果表明,PqsE 与 RhlR 的相互作用优先改变了其激活参与 PYO 产生的基因转录的能力,并提供了关于 PqsE 依赖性 RhlR 激活的新证据,突出了不同铜绿假单胞菌进化枝和菌株之间 QS 反应的可变性。
更新日期:2021-10-25
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