International Journal of Environmental Health Research ( IF 3.2 ) Pub Date : 2021-08-22 , DOI: 10.1080/09603123.2021.1969341 Subhashree Bhattacharyya 1 , Sujata Law 1
ABSTRACT
Exposure to N-nitroso compounds (NOCs) in our environment via pesticides, tobacco, and smoked meat can be potentially carcinogenic. The induction of N-N’ ethylnitrosourea (ENU), a genotoxic NOC, leads to leukemogenesis. The study aimed to explore the ameliorating effect of the Ayurvedic herb Eclipta alba on the bone marrow cells of ENU-induced leukemic mice. Eclipta alba is investigated for its anti-cancer effect on various cell lines, but never on haematological malignant models. Theefficacy of the extract was explored on leukemia by changes in body weight, survivability, peripheral blood hemogram, bone marrow cytological, histological, and cell culture studies pre-and post-treatment. The treated group revealed significant immunomodulation of the expressional profile of NF-kB family and IL-1β in marrow cells, by flow-cytometry, and immunofluorescence study. Through our experimental endeavour we depicted the cellular mechanism, signaling modality and tried to establish the anti-cancer potency of Eclipta alba on ENU-induced leukemia.
中文翻译:
旱莲草在小鼠模型中环境污染物 ENU 诱导白血病 NF-kB 信号传导的改善
摘要
通过杀虫剂、烟草和熏肉在我们的环境中接触 N-亚硝基化合物 (NOC) 可能会致癌。N-N' 乙基亚硝基脲 (ENU) 的诱导,一种基因毒性 NOC,导致白血病发生。本研究旨在探讨阿育吠陀旱莲草对 ENU 诱导的白血病小鼠骨髓细胞的改善作用。旱莲草研究了其对各种细胞系的抗癌作用,但从未对血液恶性模型进行过研究。通过治疗前后体重、存活率、外周血血象、骨髓细胞学、组织学和细胞培养研究的变化来探索提取物对白血病的功效。通过流式细胞术和免疫荧光研究,治疗组显示出骨髓细胞中 NF-kB 家族和 IL-1β 表达谱的显着免疫调节。通过我们的实验努力,我们描述了细胞机制、信号传导方式,并试图确定旱莲草对 ENU 诱导的白血病的抗癌效力。