This study was carried out with the objective to identify function prediction of novel microRNAs (miRNAs) in immature boar Sertoli cells (SCs) treated with 5-aminoimidazole-4-carboxamide-1-β-D-ribofuranoside (AICAR), which is an agonist of adenosine monophosphate-activated protein kinase (AMPK) for regulating cellular energy homeostasis. Two small RNA libraries (control and AICAR treatment) prepared from immature boar SCs were constructed and sequenced by the Illumina small RNA deep sequencing. We identified 77 novel miRNAs and predicted 177 potential target genes for 26 differential novel miRNAs (four miRNAs up-regulation and 22 miRNAs down-regulation) in AICAR-treated SCs. Gene Ontology enrichment and Kyoto Encyclopedia of Genes and Genomes pathway suggested that target genes of differential novel miRNAs were implicated in many biological processes and metabolic pathways. Our findings provided useful information for the functional regulation of novel miRNAs and target mRNAs on AMPK-activated immature boar SCs.

中文翻译：

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未成年公猪单磷酸腺苷激活蛋白激酶激活支持细胞中新型微小RNA的鉴定及功能预测

进行这项研究的目的是确定用 5-氨基咪唑-4-甲酰胺-1-β-D-呋喃核糖苷 (AICAR) 处理的未成熟公猪支持细胞 (SCs) 中新型 microRNA (miRNA) 的功能预测，这是一种腺苷一磷酸激活蛋白激酶 (AMPK) 的激动剂，用于调节细胞能量稳态。构建了两个从未成熟的公猪 SC 制备的小 RNA 文库（对照和 AICAR 处理），并通过 Illumina 小 RNA 深度测序对其进行了测序。我们鉴定了 77 种新的 miRNA，并预测了 AICAR 处理的 SC 中 26 种不同的新 miRNA（4 种 miRNA 上调和 22 种 miRNA 下调）的 177 个潜在靶基因。基因本体论富集和京都基因和基因组百科全书途径表明，差异新型 miRNA 的靶基因涉及许多生物过程和代谢途径。我们的研究结果为新型 miRNA 和靶 mRNA 在 AMPK 激活的未成熟公猪 SC 上的功能调节提供了有用的信息。