Triclocarban (TCC), an antimicrobial compound commonly added to a wide range of household and personal hygiene care products, is one of the most prevalent endocrine-disrupting substances (EDS). This study was conducted to elucidate whether in utero and lactational exposure to TCC could adversely affect folliculogenesis and the onset of puberty in female rat offspring. Twenty pregnant Sprague Dawley rats were equally divided into Control and TCC dam groups (supplemented daily with drinking water enriched with 0.5 mg/L of TCC) from gestational day5 to postnatal day21 (PND21). Female offspring, 20 from control and 20 from TCC dams, were subdivided into 4 subgroups (PND21, PND28, PND35 & PND42). The day of vaginal opening and first estrous cycle were determined. Ovarian sections of the offspring were processed for H&E staining and for immunohistochemical expression of Ki67, Caspase-3 and androgen receptors (AR) on the granulosa cells of ovarian follicles. Follicular count and atretic index were assessed besides, serum estradiol, progesterone, FSH and LH, C-reactive protein (CRP), malondialdehyde (MDA) and total antioxidant capacity (TAC) were measured. TCC offspring exhibited a significant delay in the onset of puberty and impedance of normal transition of the primordial follicles to more developed ones with altered cyctoarchitecture. Also, TCC decreased follicular count, proliferation and gonado-somatic index while it increased atretic index, apoptosis and AR of the granulosa cells along with disturbance of the feminine hormonal profile and oxidant/antioxidant balance. This study highlighted the potential long-term consequences of in utero and lactational exposure to TCC on the postnatal development of the ovary in rat offspring.

三氯卡班 (TCC) 是一种抗菌化合物，通常添加到各种家庭和个人卫生护理产品中，是最普遍的内分泌干扰物质 (EDS) 之一。本研究旨在阐明子宫内和哺乳期接触 TCC 是否会对雌性大鼠后代的卵泡发生和青春期开始产生不利影响。从妊娠第 5 天到出生后第 21 天 (PND21)，将 20 只怀孕的 Sprague Dawley 大鼠平均分为对照组和 TCC 水坝组（每天补充富含 0.5 mg/L TCC 的饮用水）。雌性后代，来自对照的 20 只和来自 TCC 水坝的 20 只，被细分为 4 个亚组（PND21、PND28、PND35 和 PND42）。确定阴道开口和第一个动情周期的日期。对后代的卵巢切片进行 H& 卵巢滤泡颗粒细胞上 Ki67、Caspase-3 和雄激素受体 (AR) 的 E 染色和免疫组织化学表达。测定卵泡计数和闭锁指数，测定血清雌二醇、孕酮、FSH和LH、C反应蛋白（CRP）、丙二醛（MDA）和总抗氧化能力（TAC）。TCC 后代表现出青春期开始的显着延迟和原始卵泡正常过渡到具有改变的细胞结构的更发达卵泡的阻抗。此外，TCC 降低了卵泡计数、增殖和性腺体细胞指数，同时增加了颗粒细胞的闭锁指数、凋亡和 AR，同时扰乱了女性激素谱和氧化剂/抗氧化剂平衡。