This study combined conventional epidemiology of human astroviruses. From 2010 to 2016, 232 stool samples from children under 5 years of age were screened using NGS and conventional RT-PCR followed by genetic analysis in order to investigate the genotypic diversity of classical human astrovirus (HAstV) circulating in Tocantins State, Brazil. HAstV was detected in 16 cases (6.9%). Seven specimens (43.7%; 7/16) were positive according RT-PCR and next-generation sequencing (NGS) to investigate the molecular to both NGS and RT-PCR. NGS and RT-PCR individually revealed six (37.5%; 6/16) and three (18.8%; 3/16) additional positive samples, respectively. Sequencing of the HAstV-positive samples revealed HAstV-1a (9/16), HAstV-4c (3/16), and HAstV-5c (4/16) lineages.

这项研究结合了人类星状病毒的常规流行病学。从 2010 年到 2016 年，为了研究在巴西托坎廷斯州流行的经典人类星状病毒 (HAstV) 的基因型多样性，使用 NGS 和常规 RT-PCR 筛选了 232 份 5 岁以下儿童的粪便样本，然后进行了遗传分析。在 16 例 (6.9%) 中检测到 HAstV。根据 RT-PCR 和下一代测序 (NGS) 研究 NGS 和 RT-PCR 的分子，七个样本 (43.7%；7/16) 呈阳性。NGS 和 RT-PCR 分别显示六个（37.5%；6/16）和三个（18.8%；3/16）额外的阳性样本。HAstV 阳性样本的测序揭示了 HAstV-1a (9/16)、HAstV-4c (3/16) 和 HAstV-5c (4/16) 谱系。