The purpose of this study was to examine the effect of prolonged every-other day (EOD) feeding on bone trace elements. Four-week old C57BL/6 female (n = 12) and male (n = 12) mice were employed in this experiment. Animals were assigned to four groups: ad libitum-AL (males and females), EOD fed (males, females). After 9 months, the mice were sacrificed. Long bones (humerus and radius) were isolated and prepared for analysis using inductively coupled plasma optical emission spectrometry to determine the Fe, Zn, Mo, Co, Cu, Mn, Cr contents. Estimation of cathepsin K expression on bone slides was performed to determine the activity of osteoclasts in bones of EOD- and AL-fed animals. Higher content of Fe in EOD-fed females compared to AL-fed females was found. In EOD-fed males, a significantly higher amount of Mo (p < 0.005) and Co (p < 0.05) in comparison to AL-fed males was noted. Gender differences in amounts of trace elements in control AL-fed males vs. females were observed. EOD feeding influences the amount of some trace elements in long bones of female and male C57BL/6 mice. However, this is not influenced by the activity of bone cells.

中文翻译：

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在 C57BL/6 雄性和雌性小鼠中延长隔日喂养后骨微量元素的估计。

本研究的目的是检查延长隔日 (EOD) 喂养对骨微量元素的影响。在该实验中使用了 4 周大的 C57BL/6 雌性 (n = 12) 和雄性 (n = 12) 小鼠。动物被分配到四组：ad libitum-AL（雄性和雌性）、EOD 喂养（雄性、雌性）。9个月后，处死小鼠。分离长骨（肱骨和桡骨），并准备使用电感耦合等离子体发射光谱法进行分析，以确定 Fe、Zn、Mo、Co、Cu、Mn、Cr ​​的含量。进行骨载玻片上组织蛋白酶 K 表达的估计以确定 EOD 和 AL 喂养动物骨骼中破骨细胞的活性。发现与 AL 喂养的女性相比，EOD 喂养的女性中铁的含量更高。在 EOD 喂养的雄性中，Mo (p < 0.005) 和 Co (p < 0. 05) 与 AL 喂养的雄性相比。观察到对照 AL 喂养的雄性与雌性在微量元素含量方面的性别差异。EOD 喂养影响雌性和雄性 C57BL/6 小鼠长骨中一些微量元素的含量。然而，这不受骨细胞活性的影响。