A statistical simplex centroid design methodology was applied to determine the effects of different solvents and their mixtures on the yield, total polyphenol content, 2′2-dipheny-l-picrylhydrazyl (DPPH) radical scavenging activity, and ferric reducing antioxidant power (FRAP) of extracts from the waste of Cannabis sativa. The different extractor solvents (ethanol, methanol, water, and hexane) and their binary and ternary combinations were evaluated. The experimental results and their response surface models showed that the highest TPC yield values occur with the binary interaction between water and ethanol around the proportion of (ethanol, 70%; water, 30%). The desirability function showed that the optimal conditions were for TPC extraction ternary mixtures which consisted of 75% ethanol, 12.5% methanol, and 12.5% water. Ternary mixtures including water and binary mixture (ethanol 50% to 75%) yielded extracts with the best DPPH antioxidant activity, whereas pure methanol was the best solvent for extracting molecules with FRAP antioxidant capacity. The desirability function including all responses showed that the optimal solvent mixture consisted of 25% ethanol and 75% methanol.

中文翻译：

---

多变量优化在摩洛哥大麻废料中酚类化合物和抗氧化剂提取中的应用

应用统计单纯形质心设计方法来确定不同溶剂及其混合物对产率、总多酚含量、2'2-二苯-l-苦基肼 (DPPH) 自由基清除活性和铁还原抗氧化能力 (FRAP) 的影响大麻废料的提取物. 评估了不同的萃取溶剂（乙醇、甲醇、水和己烷）及其二元和三元组合。实验结果及其响应面模型表明，最高的 TPC 产率值出现在水和乙醇之间的二元相互作用（乙醇，70%；水，30%）左右。合意性函数表明，TPC 提取三元混合物的最佳条件是由 75% 的乙醇、12.5% 的甲醇和 12.5% 的水组成。包括水和二元混合物（乙醇 50% 至 75%）的三元混合物产生具有最佳 DPPH 抗氧化活性的提取物，而纯甲醇是提取具有 FRAP 抗氧化能力的分子的最佳溶剂。