Modulating the microbiota is a leading-edge strategy for the restoration and maintenance of a healthy, balanced environment. The use of health-promoting bacteria has demonstrated some potential benefits as an alternative for skin microbiota intervention. Here, we investigate the manipulation of mice skin microbiota using B. subtilis incorporated into a supportive Pluronic F-127 hydrogel formulation. The formula plays an important role in delivering the bacteria to the desired action site. The B. subtilis challenge induced a shift in the composition and abundance of the skin microbiota. Containment of B. subtilis in the Pluronic F-127 hydrogel accelerated bacterial modulation compared with free B. subtilis. The abundance of both Staphylococcus and Corynebacterium spp. was altered as a result of the live bacterial intervention: the abundance of Corynebacterium increased while that of Staphylococcus decreased. Four days after last application of the B. subtilis formulation, B. subtilis counts returned to its initial level. B. subtilis intervention can induce a shift in the skin microbiota, influencing the abundance of commensal, beneficial, and pathogenic bacteria. Containment of B. subtilis in Pluronic hydrogel accelerates the microbial alteration, probably by facilitating bacterial attachment and supporting continuous growth. Our results reveal the ability of B. subtilis in Pluronic to modulate the skin microbiota composition, suggesting that the formulation holds therapeutic potential for skin disease treatment.

中文翻译：

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枯草芽孢杆菌制剂挑战后的皮肤微生物群动力学：小鼠体内研究

调节微生物群是恢复和维持健康、平衡环境的前沿策略。作为皮肤微生物群干预的替代方案，使用促进健康的细菌已经证明了一些潜在的好处。在这里，我们研究了使用加入支持性 Pluronic F-127 水凝胶配方的枯草芽孢杆菌对小鼠皮肤微生物群的操作。该配方在将细菌输送到所需的作用部位方面起着重要作用。枯草芽孢杆菌的挑战导致皮肤微生物群的组成和丰度发生变化。与游离的枯草芽孢杆菌相比，Pluronic F-127 水凝胶中包含的枯草芽孢杆菌加速了细菌调节。葡萄球菌和棒状杆菌属的丰度。由于活细菌干预而改变：枯草芽孢杆菌计数恢复到初始水平。B. subtilis 干预可以引起皮肤微生物群的变化，影响共生菌、有益菌和致病菌的丰度。Pluronic 水凝胶中含有枯草芽孢杆菌加速了微生物的改变，可能是通过促进细菌附着和支持持续生长。我们的研究结果揭示了 Pluronic 中的枯草芽孢杆菌调节皮肤微生物群组成的能力，表明该制剂具有治疗皮肤病的潜力。枯草芽孢杆菌计数恢复到初始水平。B. subtilis 干预可以引起皮肤微生物群的变化，影响共生菌、有益菌和致病菌的丰度。Pluronic 水凝胶中含有枯草芽孢杆菌加速了微生物的改变，可能是通过促进细菌附着和支持持续生长。我们的研究结果揭示了 Pluronic 中的枯草芽孢杆菌调节皮肤微生物群组成的能力，表明该制剂具有治疗皮肤病的潜力。可能是通过促进细菌附着和支持持续生长。我们的研究结果揭示了 Pluronic 中的枯草芽孢杆菌调节皮肤微生物群组成的能力，表明该制剂具有治疗皮肤病的潜力。可能是通过促进细菌附着和支持持续生长。我们的研究结果揭示了 Pluronic 中的枯草芽孢杆菌调节皮肤微生物群组成的能力，表明该制剂具有治疗皮肤病的潜力。