ABSTRACT

Objective This study aimed to analyze the influence of SLCO1B1 and APOE gene polymorphisms on coronary heart disease in Mongolian population who living in Ordos area. Methods From January 2019 to June 2020, 200 Mongolian patients with coronary heart disease admitted to our hospital and other banner hospitals were selected as the case group. At the same time, 150 randomly selected healthy Mongolian people from medical examination centers comprised the control group. The polymorphisms of SLCO1B1 (388A>G, 521 T > C) and ApoE (388 T > C, 526 C > t) were detected by real-time polymerase chain reaction. Combined with environmental data, the effect of gene polymorphism on coronary heart disease was explored. Results Both SLCO1B1 and ApoE polymorphisms satisfied Hardy-Weinberg equilibrium. The SLCO1B1 genotype *1a/*1b showed the highest frequency in the case group, accounting for 35.0%, while The SLCO1B1 genotype *1b/*1b showed the highest frequency in the control group, accounting for 32.0%. Allele *1b was the most commonly seen allele in both the case group and control group (57.8% and 53.7%, respectively). Meanwhile, The difference in the distribution of SLCO1B1 *1a/*15 genotype between the two groups was statistically significant (P < .05). Conclusion The results showed that the SLCO1B *1a/*15 genotype, ApoE ε3 /ε3 genotype, and ε3 allele reduced the risk of coronary disease in the Mongolian population, making them protective genes against this disease, while the ApoE ε4 allele increased the risk of coronary disease, making it a coronary disease risk factor.

摘要

目的分析SLCO1B1和APOE基因多态性对鄂尔多斯地区蒙古族人群冠心病的影响。方法选取2019年1月至2020年6月我院及其他三级医院收治的蒙古族冠心病患者200例作为病例组。同时，从体检中心随机抽取150名健康蒙古人作为对照组。实时聚合酶链反应检测SLCO1B1（388A>G，521T>C）和ApoE （388T>C，526C>t）的多态性。结合环境数据，探讨基因多态性对冠心病的影响。结果两者SLCO1B1和ApoE多态性满足 Hardy-Weinberg 平衡。SLCO1B1基因型*1a/*1b在病例组中频率最高，占35.0%，而SLCO1B1基因型*1b/*1b在对照组中频率最高，占32.0%。等位基因 *1b 是病例组和对照组中最常见的等位基因（分别为 57.8% 和 53.7%）。同时，两组间SLCO1B1 *1a/*15基因型分布差异有统计学意义（P < .05）。结论 结果表明，SLCO1B *1a/*15 基因型、ApoEε3 /ε3 基因型和 ε3 等位基因降低了蒙古人群患冠心病的风险，使其成为抗这种疾病的保护基因，而ApoE ε4 等位基因增加了患冠心病的风险，使其成为冠心病的危险因素。