Differences in T cell immune-related lncRNA and mRNA expression patterns between right- and left-sided colorectal cancers,Human Immunology

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Differences in T cell immune-related lncRNA and mRNA expression patterns between right- and left-sided colorectal cancers
Human Immunology ( IF 2.7 ) Pub Date : 2021-08-21 , DOI: 10.1016/j.humimm.2021.08.008
Yin Lijuan ₁ , Wu Yinhang ₂ , Wang Yangyanqiu ₃ , Hou Xiaohui ₄ , Wei Yunhai ₅ , Han Shuwen ₆

Affiliation

Background

Right-sided colorectal cancer (RCRC) and left-sided colorectal cancer (LCRC) harbor different genetic alterations associated with immune response.

Objective

This study aimed to analyze the differences in T cell immune-related RNA expression patterns between RCRC and LCRC.

Methods

The differentially expressed genes (DEGs) and lncRNAs (DElncRNAs) between LCRC and RCRC were screened from the Cancer Genome Atlas (TCGA) database. A correlation analysis between DEGs or DElncRNAs and differential T cells was also performed to obtain T cell-related genes, followed by miRNA prediction. The mRNA-lncRNA network and the competitive endogenous RNA (ceRNA) network were subsequently constructed, and the expression level of mRNA in the ceRNA network was verified using GSE104645.

Results

RCRC patients had a poorer prognosis and were older than LCRC patients. In total, 923 DEGs and 328 DElncRNAs were screened between LCRC and RCRC patients. Compared to RCRC patients, LCRC patients showed a decrease in CD8⁺ T cells. In addition, 26 miRNAs, 8 mRNAs, and 10 lncRNAs were included in the ceRNA network. Finally, the validation analysis revealed that CDHR1 and PRLR were significantly downregulated, while TRIB2 was upregulated in RCRC patients compared to LCRC patients.

Conclusion

The analysis of T cell immune-related RNA expression might provide new insights into the underlying molecular mechanisms of the differences between LCRC and RCRC.

中文翻译：

右侧和左侧结直肠癌之间 T 细胞免疫相关 lncRNA 和 mRNA 表达模式的差异

背景

右侧结直肠癌 (RCRC) 和左侧结直肠癌 (LCRC) 具有与免疫反应相关的不同基因改变。

客观的

本研究旨在分析 RCRC 和 LCRC 之间 T 细胞免疫相关 RNA 表达模式的差异。

方法

LCRC和RCRC之间的差异表达基因（DEGs）和lncRNAs（DElncRNAs）是从癌症基因组图谱（TCGA）数据库中筛选出来的。还进行了 DEG 或 DElncRNA 与差异 T 细胞之间的相关性分析，以获得 T 细胞相关基因，然后进行 miRNA 预测。随后构建了mRNA-lncRNA网络和竞争性内源性RNA（ceRNA）网络，并使用GSE104645验证了ceRNA网络中mRNA的表达水平。

结果

RCRC 患者的预后较差，并且比 LCRC 患者的年龄更大。在 LCRC 和 RCRC 患者之间总共筛选了 923 个 DEGs 和 328 个 DElncRNAs。与 RCRC 患者相比，LCRC 患者的 CD8 ⁺ T 细胞减少。此外，ceRNA 网络中包含 26 个 miRNA、8 个 mRNA 和 10 个 lncRNA。最后，验证分析显示 CDHR1 和 PRLR 显着下调，而 RCRC 患者与 LCRC 患者相比，TRIB2 上调。