Cylindrospermopsin (CYN) is a cytotoxin, and its documented effects in mammals include damage to several organs. CYN also has hormone-disrupting properties, including estrogenic activity, progesterone production inhibition, and apoptosis induction. While CYN has been reported to exert reproductive toxicity in mice, little is known about its effect on fish reproductive function. Using ex vivo organ culture, we investigated the direct action of CYN on the male reproductive system. Isolated zebrafish testis was exposed to 250, 500, and 1000 µg/L CYN for 24 h and 7 d, followed by histo-morphological analysis. The results demonstrate that exposure to CYN led to a decrease in cell types from all three phases of spermatogenesis in zebrafish testis. There were also significant changes in fshr, lhr, and igf3 transcript levels, as well as testosterone secretion following exposure to CYN. In summary, this study provides novel information on the adverse effects of CYN on testicular spermatogenesis and male reproduction in zebrafish. These results provide a framework for a better understanding of CYN toxicity and the mechanism underlying the adverse action of CYN on male reproduction in fish.

Cylindrospermopsin (CYN) 是一种细胞毒素，其对哺乳动物的影响包括对多个器官的损害。CYN 还具有干扰激素的特性，包括雌激素活性、黄体酮产生抑制和细胞凋亡诱导。虽然据报道 CYN 对小鼠产生生殖毒性，但对其对鱼类生殖功能的影响知之甚少。使用离体器官培养，我们研究了 CYN 对男性生殖系统的直接作用。将分离的斑马鱼睾丸暴露于 250、500 和 1000 µg/L CYN 24 小时和 7 天，然后进行组织形态学分析。结果表明，暴露于 CYN 导致斑马鱼睾丸中精子发生的所有三个阶段的细胞类型减少。fs hr、lhr也有显着变化和igf3转录水平，以及暴露于 CYN 后的睾酮分泌。总之，本研究提供了关于 CYN 对斑马鱼睾丸精子发生和雄性生殖的不利影响的新信息。这些结果为更好地理解 CYN 毒性和 CYN 对鱼类雄性繁殖的不利作用的机制提供了一个框架。