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Wood pellets as carriers of conidia of Trichoderma atroviride SC1 for soil application
Fungal Biology ( IF 2.5 ) Pub Date : 2021-08-21 , DOI: 10.1016/j.funbio.2021.08.003
Hamza Chammem 1 , Andrea Nesler 2 , Ilaria Pertot 3
Affiliation  

The use of biocontrol agents to control soilborne diseases is a promising alternative to chemical pesticides, however, obtaining a homogeneous distribution and incorporation of conidia of fungal biocontrol agents into the soil is often difficult. Several carriers/formulations have been proposed over time, unfortunately without offering an ultimate solution. We propose the use of wood pellets as a carrier of conidia of a saprophytic fungus that has good biodegradation and biocontrol properties (Trichoderma atroviride SC1). The coating process is based on the direct spraying of wood pellets with a conidial suspension at different rates. Beech, fir, and chestnut wood pellets were compared in terms of relevant physicochemical traits and efficacy in supporting the growth of the fungus. Beech wood pellets displayed the best characteristics in terms of water holding capacity, swelling properties, and disintegration time. T. atroviride SC1 grows best on beech and fir wood pellets and reaches a plateau after nine days of incubation, regardless of the initial coating concentrations. The addition of small quantities of a nitrogen source as tryptone or soy flour, soy proteins, and a mixture of animal proteins used as pet food to the conidial suspension can increase the growth by ten-folds on all types of wood pellets. Our results demonstrate that beech and fir wood pellets could be suitable carriers to deliver and sustain the growth of T. atroviride SC1.



中文翻译:

木颗粒作为土壤应用木霉SC1分生孢子载体

使用生物防治剂来控制土传疾病是化学农药的有希望的替代品,但是,获得均匀分布的真菌生物防治剂的分生孢子并将其掺入土壤中通常很困难。随着时间的推移,已经提出了几种载体/配方,遗憾的是没有提供最终的解决方案。我们建议使用木质颗粒作为具有良好生物降解和生物防治特性的腐生真菌 ( Trichoderma atroviride ) 的分生孢子载体。SC1)。涂层工艺基于以不同速率直接喷涂带有分生孢子悬浮液的木屑颗粒。比较了山毛榉、冷杉和栗木颗粒的相关理化特性和支持真菌生长的功效。榉木颗粒在保水性、膨胀性和崩解时间方面表现出最佳特性。T. atrovirideSC1 在山毛榉和杉木颗粒上生长最好,并且在孵化 9 天后达到平台期,无论初始涂层浓度如何。在分生孢子悬浮液中添加少量的氮源,如胰蛋白胨或大豆粉、大豆蛋白和用作宠物食品的动物蛋白混合物,可以使所有类型的木屑颗粒的生长增加十倍。我们的研究结果表明,山毛榉和杉木颗粒可能是提供和维持T. atroviride SC1 生长的合适载体。

更新日期:2021-08-21
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