Pregnancy is accompanied by significant physiological changes which can impact the health and development of the fetus and mother. Pregnancy-induced changes in plasma lipoproteins are well-documented, with modest to no impact observed on the generic measure of high-density lipoprotein (HDL) cholesterol. However, the impact of pregnancy on the concentration and composition of HDL subspecies has not been examined in depth. In this prospective study, we collected plasma from 24 non-pregnant and 19 pregnant women in their second trimester. Using nuclear magnetic resonance (NMR) we quantified 11 different lipoprotein subspecies from plasma by size, including 3 in the HDL class. We observed an increase in the number of larger HDL particles in pregnant women which were confirmed by tracking phospholipids across lipoproteins using high-resolution gel-filtration chromatography. Using liquid chromatography-mass spectrometry (LC-MS) we identified 87 lipid-associated proteins across size-speciated fractions. We report drastic shifts in multiple protein clusters across different HDL size fractions in pregnant females compared to non-pregnant controls that have major implications on HDL function. These findings significantly elevate our understanding of how changes in lipoprotein metabolism during pregnancy could impact the health of both the fetus and the mother.

中文翻译：

---

怀孕伴随着更大的高密度脂蛋白颗粒和组成不同的亚种。

怀孕伴随着显着的生理变化，这些变化会影响胎儿和母亲的健康和发育。妊娠引起的血浆脂蛋白变化已得到充分证明，对高密度脂蛋白 (HDL) 胆固醇的一般测量结果没有影响甚至没有影响。然而，尚未深入研究怀孕对 HDL 亚种浓度和组成的影响。在这项前瞻性研究中，我们收集了 24 名非妊娠妇女和 19 名妊娠中期妇女的血浆。使用核磁共振 (NMR)，我们从血浆中按大小量化了 11 种不同的脂蛋白亚种，包括 HDL 类中的 3 种。我们观察到孕妇体内较大的 HDL 颗粒数量增加，这通过使用高分辨率凝胶过滤色谱法跟踪脂蛋白中的磷脂得到证实。使用液相色谱-质谱 (LC-MS)，我们确定了 87 种不同大小的脂质相关蛋白。我们报告了与对 HDL 功能有重大影响的非妊娠对照相比，妊娠女性中不同 HDL 大小部分的多个蛋白质簇发生了剧烈变化。这些发现显着提高了我们对怀孕期间脂蛋白代谢变化如何影响胎儿和母亲健康的理解。我们报告了与对 HDL 功能有重大影响的非妊娠对照相比，妊娠女性中不同 HDL 大小部分的多个蛋白质簇发生了剧烈变化。这些发现显着提高了我们对怀孕期间脂蛋白代谢变化如何影响胎儿和母亲健康的理解。我们报告了与对 HDL 功能有重大影响的非妊娠对照相比，妊娠女性中不同 HDL 大小部分的多个蛋白质簇发生了剧烈变化。这些发现显着提高了我们对怀孕期间脂蛋白代谢变化如何影响胎儿和母亲健康的理解。