Chronic stress causes the abnormality of olfactory bulb (OB) in both anxiety and depression, however, the unique and common neurobiological underpinnings are still poorly understood. Previously, we built the three groups by chronic mild stress (CMS), depression-susceptible (Dep-Sus): with depression-like behavior, anxiety-susceptible (Anx-Sus): with anxiety-like behavior and insusceptible (Insus): without depression- and anxiety-like behaviors. To continuously explore the protein expression changes in these three groups, comparative quantitative proteomics analysis was conducted on the rat OB as crucial part of the olfactory system. Next, bioinformatics analyses were implemented whereas protein expressions were independently analyzed by parallel reaction monitoring (PRM) or Western blot (WB). The OB-proteome analysis identified totally 133 differentially expressed proteins as a CMS response. These deregulated proteins were involved in multiple functions and significant pathways potentially correlated with phenotypes of maladaptive behavior of depression or anxiety as well as adaptive behavior, and hence might act as potential candidate protein targets. The subsequent PRM-based or WB-based analyses showed that changes in Nefl, Mtmr7 and Tk2; Prkaca, Coa3, Cox6c2, Lamc1 and Tubal3; and Pabpn1, Nme3, Sos1 and Lum were uniquely associated with Dep-Sus, Anx-Sus, and Insus groups, respectively. These phenotype-specific deregulated proteins were primarily involved in multiple metabolic and signaling pathways, suggesting that the identical CMS differently impacted the olfactory protein regulation system and biological processes. To sum up, our present data as a useful proteomics underpinning provided the common and distinct molecular insights into the biochemical understanding of OB dysfunction underlying susceptibility and resiliency to chronic-stress-induced anxiety or depression.

慢性压力会导致焦虑和抑郁中的嗅球 (OB) 异常，但是，对独特和常见的神经生物学基础仍知之甚少。之前，我们通过慢性轻度压力​​（CMS）、抑郁易感（Dep-Sus）：具有抑郁样行为、焦虑易感（Anx-Sus）：具有焦虑样行为和不敏感（Insus）建立了三个组：没有类似抑郁和焦虑的行为。为了不断探索这三组中蛋白质表达的变化，对作为嗅觉系统关键部分的大鼠 OB 进行了比较定量蛋白质组学分析。接下来，实施生物信息学分析，而通过平行反应监测 (PRM) 或蛋白质印迹 (WB) 独立分析蛋白质表达。OB 蛋白质组分析总共鉴定了 133 种差异表达的蛋白质作为 CMS 响应。这些失调的蛋白质参与多种功能和重要途径，可能与抑郁或焦虑的适应不良行为以及适应性行为的表型相关，因此可能作为潜在的候选蛋白质靶标。随后基于 PRM 或基于 WB 的分析表明 Nefl、Mtmr7 和 Tk2 的变化；Prkaca、Coa3、Cox6c2、Lamc1 和 Tubal3；Pabpn1、Nme3、Sos1 和 Lum 分别与 Dep-Sus、Anx-Sus 和 Insus 组唯一相关。这些表型特异性失调蛋白主要参与多种代谢和信号通路，表明相同的 CMS 对嗅觉蛋白调节系统和生物过程的影响不同。总结，