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Transcriptome analysis reveals abscisic acid enhancing drought resistance by regulating genes related to flavonoid metabolism in pigeon pea
Environmental and Experimental Botany ( IF 5.7 ) Pub Date : 2021-08-21 , DOI: 10.1016/j.envexpbot.2021.104627
Wanlong Yang 1 , Na Li 1 , Yuxin Fan 1 , Biying Dong 1 , Zhihua Song 1 , Hongyan Cao 1 , Tingting Du 1 , Tengyue Liu 1 , Meng Qi 1 , Lili Niu 1, 2 , Dong Meng 1, 2 , Qing Yang 1, 2 , Yujie Fu 1, 2
Affiliation  

Abscisic acid (ABA) plays an important role in drought resistance of plants. Plants subjected to drought stress often produce a large number of secondary metabolites. More and more studies indicate that secondary metabolites are directly related to plant stress resistance. However, there are few reports about the influence of ABA regulating secondary metabolism on drought. In our study, we found that as a pioneer tree species in karst landforms, the drought resistance can also be improved by ABA. ABA can reduce the stomatal opening of pigeon pea under drought stress and reduce the influx of H+ from the root tip. At the same time, we found that in ABA-treated pigeon pea, flavonoid metabolites accumulate in large amounts, especially genistein, genistin and pterostilbene. Through transcriptome sequencing analysis, it was found that after ABA treatment, flavonoids biosynthesis pathways changed significantly; among them, the key enzyme genes CHS, HIDH and ROMT in the biosynthesis pathway of genistein, genistin and pterostilbene were significantly up-regulated. According to the co-expression analysis in the transcriptome data, we screened that multiple MYB and bHLH transcription factors may be involved in the regulation of flavonoid accumulation. And the biosynthetic genes contained binding sites of related transcription factors. Through RT-PCR, we further analyzed the expression of MYB, bHLH and related biosynthetic genes after ABA treatment at different times, and found that they were all regulated by ABA. By High Performance Liquid Chromatography (HPLC) analysis, we found the increase of genistein, genistin and pterostilbene content in pigeon pea treated with ABA under drought stress. Through hairy root transgenic analysis, We found that CcMYB114 regulate drought resistance by modulating the expression of CcHIDH1 and CcHIDH3, and then regulating genistein and genistin accumulation in pigeon pea. This study provides evidence to analyze the regulation of ABA in pigeon pea on the metabolism of flavonoids and thus affect its drought resistance.



中文翻译:

转录组分析揭示脱落酸通过调控木豆黄酮代谢相关基因增强抗旱性

脱落酸(ABA)在植物抗旱性中起重要作用。遭受干旱胁迫的植物通常会产生大量次生代谢物。越来越多的研究表明,次生代谢产物与植物的抗逆性直接相关。然而,关于ABA调节次生代谢对干旱影响的报道较少。在我们的研究中,我们发现作为喀斯特地貌中的先锋树种,ABA也可以提高抗旱性。ABA可减少干旱胁迫下木豆的气孔开放,减少H +的流入从根尖。同时,我们发现在ABA​​处理的木豆中,黄酮类代谢物大量积累,尤其是染料木黄酮、染料木苷和紫檀芪。通过转录组测序分析发现,ABA处理后,黄酮类生物合成途径发生显着变化;其中,染料木素、染料木素和紫檀芪生物合成途径中的关键酶基因CHS、HIDH和ROMT显着上调。根据转录组数据中的共表达分析,我们筛选出多个 MYB 和 bHLH 转录因子可能参与了类黄酮积累的调控。并且生物合成基因含有相关转录因子的结合位点。通过RT-PCR,我们进一步分析了MYB的表达,ABA处理不同时间后的bHLH及相关生物合成基因,发现均受ABA调控。通过高效液相色谱(HPLC)分析,我们发现在干旱胁迫下,ABA处理的木豆中染料木黄酮、染料木苷和紫檀芪的含量增加。通过毛根转基因分析,我们发现CcMYB114通过调节CcHIDH1和CcHIDH3的表达,进而调控木豆中染料木素和染料木素的积累来调节抗旱性。本研究为分析木豆中ABA对黄酮类物质代谢的调控进而影响其抗旱性提供了依据。干旱胁迫下 ABA 处理木豆中染料木苷和紫檀芪的含量. 通过毛根转基因分析,我们发现CcMYB114通过调节CcHIDH1和CcHIDH3的表达,进而调节木豆中染料木素和染料木素的积累来调节抗旱性。本研究为分析木豆中ABA对黄酮类物质代谢的调控进而影响其抗旱性提供了依据。干旱胁迫下 ABA 处理木豆中染料木苷和紫檀芪的含量. 通过毛根转基因分析,我们发现CcMYB114通过调节CcHIDH1和CcHIDH3的表达,进而调控木豆中染料木素和染料木素的积累来调节抗旱性。本研究为分析木豆中ABA对黄酮类物质代谢的调控进而影响其抗旱性提供了依据。

更新日期:2021-08-29
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