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Eco-friendly Bleaching of Agrowaste Wheat Straw Using Crude Alkalo-Thermotolerant Cellulase-Free Xylano-Pectinolytic Enzymes
Applied Biochemistry and Biotechnology ( IF 3 ) Pub Date : 2021-08-20 , DOI: 10.1007/s12010-021-03641-6
Divya Sharma 1, 2 , Raksha Nagpal 1 , Sharad Agrawal 1, 3 , Nishikant Bhardwaj 4 , Ritu Mahajan 1
Affiliation  

The aim of this study was to evaluate the potential of xylanase-pectinase enzymes in bleaching of wheat straw pulp, just to cut down the toxic wastes, in order to manage the environmental pollution. The appropriate parameters of bleaching were evaluated, and best conditions were xylanase and pectinase dose of 5.0 and 1.66 IU/g of pulp, respectively, along with material to liquid ratio of 1:7.5 (g/ml), temperature 55 °C, treatment time 3 h, Tween-80 1%, and pH 8.5. The release of reducing sugar and other non-cellulosic impurities, phenolic-hydrophobic-lignin was maximum at best bleaching conditions. Prebleaching of wheat straw pulp using these enzymes showed 14.75% decline in kappa number. Enzymatic bleaching plus 100% chemical bleaching also led to 27.90% reduction in yellowness. Using this methodology, the consumption of active chlorine was reduced up to 25%, along with an increase in burst index (7.98%), tear index (3.42%), breaking length (5.30%), viscosity (11.22%), gurley porosity (12.50%), and double-fold number (23.08%), which exhibits a remarkable enhancement in all the properties of pulp treated with enzymes. Microscopic images also confirm the effectiveness of enzymatic treatment in bleaching of wheat straw pulp. BOD and COD values of effluent also decreased by 20.74 and 17.87%, respectively. This research focussing on producing better grade paper using an eco-friendly approach would certainly benefit the paper and pulp industry. This is the first report, depicting bleaching capability of xylanase-pectinase enzymes for soda-anthraquinone pulp of wheat straw.



中文翻译:

使用粗制碱耐热纤维素酶的无木聚糖果胶分解酶对农业废弃物小麦秸秆进行环保漂白

本研究的目的是评估木聚糖酶-果胶酶在麦草浆漂白中的潜力,以减少有毒废物,从而控制环境污染。评估了漂白的合适参数,最佳条件为木聚糖酶和果胶酶剂量分别为 5.0 和 1.66 IU/g 纸浆,料液比为 1:7.5 (g/ml),温度 55 ℃,处理时间 3 小时,Tween-80 1%,pH 8.5。在最佳漂白条件下,还原糖和其他非纤维素杂质、酚-疏水-木质素的释放量最大。使用这些酶对麦草浆进行预漂白时,卡伯值下降了 14.75%。酶促漂白加 100% 化学漂白也导致黄度降低 27.90%。使用这种方法,活性氯消耗量减少高达25%,同时爆破指数(7.98%)、撕裂指数(3.42%)、断裂长度(5.30%)、粘度(11.22%)、格利孔隙率(12.50%)增加, 和双倍数 (23.08%), 表明酶处理纸浆的所有性能都有显着提高。显微图像也证实了酶处理在小麦草浆漂白中的有效性。出水的BOD和COD值也分别下降了20.74和17.87%。这项专注于使用环保方法生产更高等级纸张的研究肯定会使造纸和纸浆行业受益。这是第一份报告,描述了木聚糖酶-果胶酶对麦秸苏打-蒽醌浆的漂白能力。断裂长度 (5.30%)、粘度 (11.22%)、格莱孔隙率 (12.50%) 和倍数 (23.08%),酶处理纸浆的所有性能均显着提高。显微图像也证实了酶处理在小麦草浆漂白中的有效性。出水的BOD和COD值也分别下降了20.74和17.87%。这项专注于使用环保方法生产更高等级纸张的研究肯定会使造纸和纸浆行业受益。这是第一份报告,描述了木聚糖酶-果胶酶对麦秸苏打-蒽醌浆的漂白能力。断裂长度 (5.30%)、粘度 (11.22%)、格莱孔隙率 (12.50%) 和倍数 (23.08%),酶处理纸浆的所有性能均显着提高。显微图像也证实了酶处理在小麦草浆漂白中的有效性。出水的BOD和COD值也分别下降了20.74和17.87%。这项专注于使用环保方法生产更高等级纸张的研究肯定会使造纸和纸浆行业受益。这是第一份报告,描述了木聚糖酶-果胶酶对麦秸苏打-蒽醌浆的漂白能力。显微图像也证实了酶处理在小麦草浆漂白中的有效性。出水的BOD和COD值也分别下降了20.74和17.87%。这项专注于使用环保方法生产更高等级纸张的研究肯定会使造纸和纸浆行业受益。这是第一份报告,描述了木聚糖酶-果胶酶对麦秸苏打-蒽醌浆的漂白能力。显微图像也证实了酶处理在小麦草浆漂白中的有效性。出水的BOD和COD值也分别下降了20.74和17.87%。这项专注于使用环保方法生产更高等级纸张的研究肯定会使造纸和纸浆行业受益。这是第一份报告,描述了木聚糖酶-果胶酶对麦秸苏打-蒽醌浆的漂白能力。

更新日期:2021-08-20
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