Human T-lymphotropic virus type 1 (HTLV-1) was the first human retrovirus described. The viral factors involved in the different clinical manifestations of infected individuals are still unknown, and in this sense, sequencing technologies can support viral genome studies, contributing to a better understanding of infection outcome. Currently, several sequencing technologies are available with different approaches. To understand the methodological advances in the HTLV-1 field, it is necessary to organize a synthesis by a rigorous review. This systematic literature review describes different technologies used to generate HTLV-1 sequences. The review follows the PRISMA guidelines, and the search for articles was performed in PubMed, Lilacs, Embase, and SciELO databases. From the 574 articles found in search, 62 were selected. The articles showed that, even with the emergence of new sequencing technologies, the traditional Sanger method continues to be the most commonly used methodology for generating HTLV-1 genome sequences. There are many questions that remain unanswered in the field of HTLV-1 research, and this reflects on the small number of studies using next-generation sequencing technologies, which could help address these gaps. The data compiled and analyzed here can help research on HTLV-1, assisting in the choice of sequencing technologies.

人类 T 淋巴细胞病毒 1 型 (HTLV-1) 是第一个描述的人类逆转录病毒。感染个体不同临床表现所涉及的病毒因素仍然未知，从这个意义上说，测序技术可以支持病毒基因组研究，有助于更好地了解感染结果。目前，几种测序技术可用于不同的方法。要了解 HTLV-1 领域的方法学进展，有必要通过严格的审查来组织综合。这篇系统的文献综述描述了用于生成 HTLV-1 序列的不同技术。审查遵循 PRISMA 指南，并在 PubMed、Lilacs、Embase 和 SciELO 数据库中搜索文章。从搜索到的 574 篇文章中，选择了 62 篇。文章表明，即使出现了新的测序技术，传统的 Sanger 方法仍然是生成 HTLV-1 基因组序列的最常用方法。在 HTLV-1 研究领域仍有许多问题没有得到解答，这反映了使用下一代测序技术的少数研究，这可能有助于解决这些差距。这里编译和分析的数据可以帮助对HTLV-1的研究，辅助测序技术的选择。这反映了使用下一代测序技术的少数研究，这可能有助于解决这些差距。这里编译和分析的数据可以帮助对HTLV-1的研究，辅助测序技术的选择。这反映了使用下一代测序技术的少数研究，这可能有助于解决这些差距。这里编译和分析的数据可以帮助对HTLV-1的研究，辅助测序技术的选择。