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Molecular characterization, expression, and apoptosis regulation of siva1 in protogynous hermaphrodite fish ricefield eel (Monopterus albus)
Fish Physiology and Biochemistry ( IF 2.9 ) Pub Date : 2021-08-20 , DOI: 10.1007/s10695-021-00997-8
Zhi He 1 , Faqiang Deng 1 , Zhijun Ma 1 , Qian Zhang 1 , Jiayang He 1 , Lijuan Ye 1 , Hongjun Chen 1 , Deying Yang 1 , Liang He 1 , Jie Luo 1 , Taiming Yan 1
Affiliation  

Siva1, which induces extensive apoptosis, has been well characterized. To elucidate the molecular function of Siva1 in ricefield eel, molecular characterization and phylogenetic analysis were performed, and the mRNA expression in the ovary at different developmental stages and ovary tissues exposed to H2O2 and Z-VAD-FMK in vitro were also evaluated. The results indicated that ricefield eel Siva1 was highly conserved and contains three conserved motifs, despite 83 amino acid differences upstream of the initiation codon. Phylogenetic analysis demonstrated that ricefield eel Siva1 clusters together with the Siva1 protein of the other fish, with high sequence homology with that of Lates calcarifer. Quantitative real-time polymerase chain reaction analysis showed high siva1 expression levels in the ovary and low expression levels in the liver. The higher mRNA levels of siva1 were detected in the IE and IM, and the lower siva1 mRNA levels were found in the OM, IL, and TE during gonadal development. Additionally, siva1 expression levels in the ovarian tissues were significantly increased at 1 h post incubation (hpi) with H2O2 and then significantly decreased at 2 hpi; however, siva1 expression was upregulated significantly at 4 and 8 hpi, similar to the patterns observed with caspase3, which was used as a molecular marker of apoptosis. Moreover, the siva1 mRNAs were elevated significantly than that in control groups at 1 hpi, but the expression of siva1 was down-regulated dramatically at 2, 4, and 8 hpi, which were similar with that of caspase3 expression profiles after Z-VAD-FMK incubation. What’s more, Pearson’s correlation coefficients showed strongly positive relationships between siva1 and caspase3. These findings suggest that Siva1 plays an important apoptosis role in gonadal development of ricefield eel.



中文翻译:

雌雄同体稻田鳗(Monopterus albus)中siva1的分子特征、表达和凋亡调控

诱导广泛细胞凋亡的 Siva1 已得到很好的表征。为了阐明Siva1在稻田鳗中的分子功能,进行了分子表征和系统发育分析,并评估了体外暴露于H 2 O 2和Z-VAD-FMK的卵巢不同发育阶段和卵巢组织中的mRNA表达. 结果表明,尽管起始密码子上游有 83 个氨基酸差异,但稻田鳗 Siva1 高度保守并包含三个保守基序。系统发育分析表明,稻鳗Siva1与其他鱼类的Siva1蛋白聚集在一起,与Lates calcarifer的序列同源性高. 定量实时聚合酶链反应分析显示卵巢中siva1表达水平高,肝脏中表达水平低。的较高的mRNA水平siva1在IE和IM进行检测,并且下siva1性腺发育过程中在OM,IL和TE被发现的mRNA水平。此外,卵巢组织中siva1 的表达水平在 H 2 O 2孵育后 1 小时 (hpi) 显着增加,然后在 2 hpi 显着降低;然而,siva1表达在 4 和 8 hpi 显着上调,类似于使用caspase3观察到的模式,用作细胞凋亡的分子标志物。此外,siva1 mRNAs在1 hpi时比对照组显着升高,但siva1的表达在2、4和8 hpi时显着下调,这与Z-VAD后的caspase3表达谱相似- FMK 孵化。更重要的是,Pearson 相关系数显示siva1caspase3之间存在很强的正相关关系这些发现表明 Siva1 在稻鳗性腺发育中起着重要的细胞凋亡作用。

更新日期:2021-08-20
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