We developed a hemocompatible, bio-inspired, multivalent, polymeric-chelating assembly based on the poly(2-methacryloyloxyethyl phosphorylcholine)-b-poly(serinyl acrylate) (PMPC-b-PserA) zwitterionic diblock copolymer. Functional PMPC-b-PserA was synthesized via reversible addition-fragmentation chain transfer (RAFT) polymerization to catch and encapsulate free copper ions (Cu²⁺) in a solution. PMPC with an identical polar group to phospholipids exhibits high hydrophilicity and fouling resistance against non-specific adsorption, and inertness to the metal ions. On the other hand, PserA with pendant groups of amino acids possesses a strong capability to react with Cu²⁺ by coordination interaction. Therefore, when PMPC-b-PserA was brought into contact with Cu²⁺, a hydrophobic core with multiple coordination “bridges” between polymers and Cu²⁺ was formed, leading to self-assembly of core-shell polymer-metal nanoparticles. As a result, free Cu²⁺ ions can be removed from the solution to prevent damage to cells and tissues. The synthesis and chemical structure of PMPC-b-PserA were characterized, and the formation of self-assembled polymer-Cu²⁺ nanoparticles and colloidal stability were analyzed. More importantly, the detoxification of PMPC-b-PserA in presence of Cu²⁺ with fibroblast cells was demonstrated by increased cell viability >80%. In addition, the hemolysis, which occurred due to disruption of RBC membranes by free Cu²⁺, was effectively suppressed by adding PMPC-b-PserA. The bio-inspired and biocompatible chelating agent of PMPC-b-PserA provides a new treatment approach to encapsulate and detoxify heavy metals in complex media for chelation therapy.

我们开发了一种基于聚（2-甲基丙烯酰氧基乙基磷酸胆碱）-b-聚（丙烯酸丝氨酸酯）（PMPC-b-PserA）两性离子二嵌段共聚物的血液相容性、仿生、多价、聚合螯合组件。功能性 PMPC-b-PserA 是通过可逆加成-断裂链转移 (RAFT) 聚合合成的，以捕获和封装溶液中的游离铜离子 (Cu ²⁺ )。与磷脂具有相同极性基团的 PMPC 表现出高亲水性和抗非特异性吸附抗污性，以及对金属离子的惰性。另一方面，带有氨基酸侧基的 PserA 具有通过配位相互作用与 Cu ²⁺反应的强大能力。因此，当 PMPC-b-PserA 与 Cu ²⁺接触时，在聚合物和Cu ²⁺之间形成具有多个配位“桥”的疏水核，导致核-壳聚合物-金属纳米颗粒的自组装。因此，可以从溶液中去除游离的 Cu ²⁺离子，以防止对细胞和组织造成损害。表征了PMPC-b-PserA的合成和化学结构，并分析了自组装聚合物-Cu ²⁺纳米粒子的形成和胶体稳定性。更重要的是，PMPC-b-PserA 在 Cu ²⁺存在下对成纤维细胞的解毒作用通过细胞活力增加 > 80% 来证明。此外，由于游离 Cu ²⁺破坏红细胞膜而发生的溶血, 通过添加 PMPC-b-PserA 被有效抑制。PMPC-b-PserA 的仿生和生物相容性螯合剂提供了一种新的治疗方法，可在复杂介质中封装和解毒重金属以进行螯合治疗。