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Multi-scale ensemble properties of the Escherichia coli RNA degradosome
Molecular Microbiology ( IF 3.6 ) Pub Date : 2021-08-20 , DOI: 10.1111/mmi.14800
Tom Dendooven 1 , Giulia Paris 1 , Alexander V Shkumatov 2, 3 , Md Saiful Islam 1 , Alister Burt 4 , Marta A Kubańska 1 , Tai Yuchen Yang 1 , Steven W Hardwick 1 , Ben F Luisi 1
Affiliation  

In organisms from all domains of life, multi-enzyme assemblies play central roles in defining transcript lifetimes and facilitating RNA-mediated regulation of gene expression. An assembly dedicated to such roles, known as the RNA degradosome, is found amongst bacteria from highly diverse lineages. About a fifth of the assembly mass of the degradosome of Escherichia coli and related species is predicted to be intrinsically disordered – a property that has been sustained for over a billion years of bacterial molecular history and stands in marked contrast to the high degree of sequence variation of that same region. Here, we characterize the conformational dynamics of the degradosome using a hybrid structural biology approach that combines solution scattering with ad hoc ensemble modelling, cryo-electron microscopy, and other biophysical methods. The E. coli degradosome can form punctate bodies in vivo that may facilitate its functional activities, and based on our results, we propose an electrostatic switch model to account for the propensity of the degradosome to undergo programmable puncta formation.

中文翻译:

大肠杆菌 RNA 降解体的多尺度集合特性

在来自生命各个领域的生物体中,多酶组装在定义转录物寿命和促进 RNA 介导的基因表达调节方面发挥着核心作用。在来自高度多样化谱系的细菌中发现了一个专门用于此类角色的组件,称为 RNA 降解体。约五分之一的大肠杆菌降解体组装质量据预测,相关物种本质上是无序的——这种特性已经持续了超过 10 亿年的细菌分子历史,与同一区域的高度序列变异形成鲜明对比。在这里,我们使用混合结构生物学方法来表征降解体的构象动力学,该方法将溶液散射与临时集成建模、低温电子显微镜和其他生物物理方法相结合。大肠杆菌降解体可以在体内形成点状体,这可能有助于其功能活动,根据我们的结果,我们提出了一个静电开关模型来解释降解体进行可编程斑点形成的倾向。
更新日期:2021-08-20
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