Abstract

Fibroblast growth factor 2 (FGF-2) is a multifunctional protein that has major roles in wound healing, tissue repair, and regeneration. This therapeutic protein is widely used for burn treatment because it can stimulate cell proliferation and differentiation, angiogenesis, and extracellular matrix remodeling. In this study, we developed a simple method using a controlled heated brass rod to create a homogenous third-degree burn murine model and evaluated the treatment using recombinant human FGF-2 (rhFGF-2). The results indicated that the wound area was 0.83 ± 0.05 cm² and wound depth was 573.42 ± 147.82 μm. Mice treated with rhFGF-2 showed higher rates of wound closure, granulation tissue formation, angiogenesis, and re-epithelialization than that of phosphate-buffered saline (PBS)-treated group. In conclusion, our lab-made rhFGF-2 could be a potentially therapeutic protein for burn treatment as well as a bioequivalent drug for other commercial applications using FGF-2.

摘要

成纤维细胞生长因子 2 (FGF-2) 是一种多功能蛋白，在伤口愈合、组织修复和再生中具有重要作用。这种治疗性蛋白质广泛用于烧伤治疗，因为它可以刺激细胞增殖和分化、血管生成和细胞外基质重塑。在这项研究中，我们开发了一种简单的方法，使用受控的加热黄铜棒创建同质三度烧伤小鼠模型，并评估使用重组人 FGF-2 (rhFGF-2) 的治疗。结果表明伤口面积为0.83±0.05 cm ²伤口深度为 573.42 ± 147.82 μm。与磷酸盐缓冲盐水 (PBS) 处理组相比，用 rhFGF-2 处理的小鼠显示出更高的伤口闭合率、肉芽组织形成、血管生成和再上皮化。总之，我们实验室制造的 rhFGF-2 可能是用于烧伤治疗的潜在治疗性蛋白质，以及使用 FGF-2 的其他商业应用的生物等效药物。