The use of botanical dietary supplements for the alleviation of conditions such as hot flashes, premenstrual syndrome, and fertility is prolific worldwide. Estrogen and progesterone receptors (ER and PR) and their corresponding steroid hormones are critical for the relief of hot flashes and the treatment of patients who develop endometriosis, and these pathways can influence the development of endometrial, ovarian, and breast cancers. However, few studies have investigated or identified the natural product components in herbal supplements that act on the PR. In the current study, a new secoiridoid, demethoxy-cornuside (1), along with six known secoiridoids (2–7) were isolated from the twigs of dogwood (Cornus officinalis) by bioassay-guided isolation with a progesterone response element (PRE)/luciferase (Luc) reporter assay in Ishikawa cells. Four phytoprogestins (1, 2, 6, 7) potentiated the effect of progesterone in the PRE/Luc assay. This study demonstrates that C. officinalis components might potentiate progesterone signaling in the presence of progesterone, which could modify progesterone receptor action in hormone-responsive tissues such as the uterus and mammary gland.

中文翻译：

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山茱萸 (Cornus officinalis) 中的 Secoiridoids 增强黄体酮信号传导

使用植物性膳食补充剂来缓解潮热、经前期综合征和生育能力等病症在世界范围内是多产的。雌激素和孕激素受体（ER 和 PR）及其相应的类固醇激素对于缓解潮热和治疗发生子宫内膜异位症的患者至关重要，这些途径可影响子宫内膜癌、卵巢癌和乳腺癌的发展。然而，很少有研究调查或确定草药补充剂中对 PR 起作用的天然产品成分。在目前的研究中，从山茱萸（Cornus officinalis) 通过在石川细胞中使用黄体酮反应元件 (PRE)/荧光素酶 (Luc) 报告基因测定进行生物测定指导的分离。四种植物孕激素（1、2、6、7 ）增强了孕酮在 PRE/Luc 测定中的作用。这项研究表明，在黄体酮存在的情况下， C. officinalis成分可能会增强黄体酮信号传导，这可能会改变黄体酮受体在激素反应组织（如子宫和乳腺）中的作用。