L-alanine is extensively used in chemical, food, and medicine industries. Industrial production of L-alanine has been mainly based on the enzymatic process using petroleum-based L-aspartic acid as the substrate. L-alanine production from renewable biomass using microbial fermentation process is an alternative route. Many microorganisms can naturally produce L-alanine using aminotransferase or L-alanine dehydrogenase. However, production of L-alanine using the native strains has been limited due to their low yields and productivities. In this review, metabolic engineering of microorganisms for L-alanine production was summarized. Among them, the Escherichia coli strains developed by Dr. Lonnie Ingram's group which can produce L-alanine with anaerobic fermentation process had several advantages, especially having high L-alanine yield, and it was the first one that realized commercialization. L-alanine is also the first amino acid that could be industrially produced by anaerobic fermentation.

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L-丙氨酸生产微生物代谢工程

L-丙氨酸广泛应用于化工、食品和医药工业。L-丙氨酸的工业生产主要基于以石油基L-天冬氨酸为底物的酶法生产。使用微生物发酵工艺从可再生生物质生产 L-丙氨酸是另一种途径。许多微生物可以利用转氨酶或L-丙氨酸脱氢酶自然产生L-丙氨酸。然而，由于其低产率和生产率，使用天然菌株生产 L-丙氨酸受到限制。在这篇综述中，总结了用于 L-丙氨酸生产的微生物代谢工程。其中，Lonnie Ingram博士课题组开发的厌氧发酵生产L-丙氨酸的大肠杆菌菌株具有多项优点，特别是L-丙氨酸产量高，是第一个实现商业化的菌株。L-丙氨酸也是第一个可以通过厌氧发酵工业化生产的氨基酸。