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Response Surface Methodology to Optimize Supercritical Carbon Dioxide Extraction of Polygonum cuspidatum
Journal of AOAC INTERNATIONAL ( IF 1.6 ) Pub Date : 2021-08-17 , DOI: 10.1093/jaoacint/qsab108
Ningjie Ruan 1 , Zhen Jiao 1, 2, 3 , Linglong Tang 3, 4
Affiliation  

Background Resveratrol and emodin are abundant polyhydroxy compounds that are found in the Chinese traditional medicinal herb Polygonum cuspidatum and widely used due to their excellent antioxidant properties. Objective This study aimed to obtain the maximum extraction yields of resveratrol and emodin from P. cuspidatum via green extraction. Methods The extracts were acquired through supercritical carbon dioxide (SC-CO2) extraction with ethanol as cosolvent. The independent variables of the extraction process, namely temperature (45–55°C), pressure (20–30 MPa), and ethanol content (80–120 mL/L), were optimized by response surface methodology. Results These variables had marked effects on the yields of resveratrol and emodin. Second-order polynomial mathematical models were developed and applied to predict the optimal extraction conditions (i.e., temperature of 51.8°C, pressure of 25.34 MPa, and ethanol content of 110.83 mL/L). Under these conditions, confirmatory experiments showed that the yields of resveratrol and emodin were 2.564 ± 0.121 and 2.804 ± 0.108 mg/g, respectively. High antioxidant properties, strong free radical scavenging abilities, and good reducing abilities were observed for the extracts. Conclusion Resveratrol and emodin can be successfully extracted from P. cuspidatum by SC-CO2, and RSM can optimize the process. Highlights The SC-CO2 extraction of resveratrol and emodin from P. cuspidatum was developed, and RSM was successfully used to optimize the extraction parameters and predict the optimal conditions.

中文翻译:

响应面法优化虎杖超临界二氧化碳萃取

背景 白藜芦醇和大黄素是在中药虎杖中发现的丰富的多羟基化合物,由于其优异的抗氧化特性而被广泛使用。目的通过绿色提取获得虎杖中白藜芦醇和大黄素的最大提取率。方法 以乙醇为助溶剂,通过超临界二氧化碳(SC-CO2)萃取获得提取物。提取过程的自变量,即温度(45-55°C)、压力(20-30 MPa)和乙醇含量(80-120 mL/L),通过响应面法进行了优化。结果这些变量对白藜芦醇和大黄素的产量有显着影响。开发并应用二阶多项式数学模型来预测最佳提取条件(即,温度 51.8°C,压力 25.34 MPa,乙醇含量 110.83 mL/L)。在这些条件下,验证性实验表明,白藜芦醇和大黄素的得率分别为 2.564 ± 0.121 和 2.804 ± 0.108 mg/g。提取物具有较高的抗氧化性能、较强的自由基清除能力和良好的还原能力。结论 SC-CO2可以成功地从虎杖中提取白藜芦醇和大黄素,RSM可以优化工艺。重点开发了SC-CO2提取虎杖中白藜芦醇和大黄素的方法,并成功利用RSM优化提取参数和预测最佳条件。验证性实验表明,白藜芦醇和大黄素的产量分别为2.564±0.121和2.804±0.108 mg/g。提取物具有较高的抗氧化性能、较强的自由基清除能力和良好的还原能力。结论 SC-CO2可以成功地从虎杖中提取白藜芦醇和大黄素,RSM可以优化工艺。重点开发了SC-CO2提取虎杖中白藜芦醇和大黄素的方法,并成功利用RSM优化提取参数和预测最佳条件。验证性实验表明,白藜芦醇和大黄素的产量分别为2.564±0.121和2.804±0.108 mg/g。提取物具有较高的抗氧化性能、较强的自由基清除能力和良好的还原能力。结论 SC-CO2可以成功地从虎杖中提取白藜芦醇和大黄素,RSM可以优化工艺。重点开发了SC-CO2提取虎杖中白藜芦醇和大黄素的方法,并成功利用RSM优化提取参数和预测最佳条件。cuspidatum 通过 SC-CO2 和 RSM 可以优化工艺。重点开发了SC-CO2提取虎杖中白藜芦醇和大黄素的方法,并成功利用RSM优化提取参数和预测最佳条件。cuspidatum 通过 SC-CO2 和 RSM 可以优化工艺。重点开发了SC-CO2提取虎杖中白藜芦醇和大黄素的方法,并成功利用RSM优化提取参数和预测最佳条件。
更新日期:2021-08-17
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