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Integrated study on subcellular localization and chemical speciation of Pb reveals root strategies for Pb sequestration and detoxification in Salix integra
Plant and Soil ( IF 4.9 ) Pub Date : 2021-08-17 , DOI: 10.1007/s11104-021-05045-1
Shufeng Wang 1 , Xiang Shi 1 , Guangcai Chen 1 , Mir Md Abdus Salam 2
Affiliation  

Background and aims

Salix integra has a high tolerance to lead (Pb) stress, and has been applied in the phytostabilization of Pb-Zn mine tailings in East China. Mechanisms for Pb sequestration and detoxification were poorly understood in Salix plants. The present study aimed to elucidate where Pb was localized and how Pb was combined in roots of S. integra for a better understanding of Pb tolerance strategies.

Methods

Histochemical methods combined with synchrotron-based X-ray fluorescence microscopy, transmission electron microscopy and fourier transform infrared spectroscopy were used to explore the distribution and chemical forms of Pb at cellular and subcellular levels in S. integra.

Results

The accumulation of Pb displayed a fast linear uptake in roots and stems in the short-term period, whereas a slower Freundlich-like uptake in leaves. Micro-XRF and histochemical staining revealed that the absorbed Pb in roots was mostly restricted in the cortex, and partly translocated in the stele. At the subcellular level, most Pb in roots was localized in the cell walls and trophoplast fraction, and the free -OH and pectin C—O/C—O—S groups may be involved in the cell wall sequestration. It was also found that the majority of Pb in roots existed as phosphate and oxalate.

Conclusions

S. integra has a high uptake potential of accumulating Pb in their roots. The absorbed Pb was primarily localized in the cell walls and combined with -OH or pectin groups, and precipitation as phosphate or oxalate may be responsible for the detoxification in root cells.



中文翻译:

铅的亚细胞定位和化学形态的综合研究揭示了积柳中铅封存和解毒的根策略

背景和目标

Salix integra对铅(Pb)胁迫具有较高的耐受性,已应用于华东地区铅锌尾矿的植物稳定化。在属植物中,人们对 Pb 封存和解毒的机制知之甚少。本研究旨在阐明 Pb 的位置以及 Pb 如何在S. integra 的根中结合,以便更好地了解 Pb 耐受策略。

方法

组织化学方法结合基于同步加速器的 X 射线荧光显微镜、透射电子显微镜和傅里叶变换红外光谱,用于探索S. integra细胞和亚细胞水平上 Pb 的分布和化学形式。

结果

Pb 的积累在短期内在根和茎中表现出快速的线性吸收,而在叶片中则表现出较慢的 Freundlich 样吸收。Micro-XRF 和组织化学染色显示根中吸收的 Pb 主要限制在皮质中,部分转移到石柱中。在亚细胞水平上,根中的大部分 Pb 定位于细胞壁和滋养体部分,游离 -OH 和果胶 C-O/C-OS 基团可能参与细胞壁的螯合。还发现根中的大部分铅以磷酸盐和草酸盐的形式存在。

结论

S. integra具有在其根部积累 Pb 的高吸收潜力。吸收的 Pb 主要位于细胞壁中,并与 -OH 或果胶基团结合,沉淀为磷酸盐或草酸盐可能是根细胞解毒的原因。

更新日期:2021-08-19
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