Soybean mosaic virus (SMV) is a member of genus Potyvirus, which causes worldwide soybean [Glycine max (L.) Merr.] yield loss and seed quality deterioration. It is of great significance to find new resistance loci and genes for cultivation of soybean variety. In the present study, a recombinant inbred line (RIL) population and a genome-wide association study (GWAS) panel, which contained 193 lines and 379 germplasms, respectively, were used for QTL mapping of resistance to SMV. Linkage mapping identified a major QTL, qSMV13, on chromosome 13, conferring resistance to SMV SC3 and SC7 strains, explaining phenotypic variations 71.21 and 76.59 %, respectively. The QTL qSMV13 was located close to the known SMV resistance loci Rsv1-h. GWAS analysis revealed five single nucleotide polymorphisms (SNPs) significantly associated with resistance to SC3 on chromosomes 2, 11, 13, 14 and 16. One of the SNP markers, ss715614844, was the right flanking marker of qSMV13. Combining linkage mapping and GWAS analysis enabled us to delimit qSMV13 in a 97.2-kb genomic region containing seven genes. A LRR-RLK protein was proposed as the candidate gene of qSMV13. These results provided selection markers and candidate genes for SMV resistance in soybean molecular breeding programs.

大豆花叶病毒(SMV)是Potyvirus属的一员，它导致全球大豆[ Glycine max (L.) Merr.]产量损失和种子质量恶化。寻找新的抗性位点和基因对大豆品种培育具有重要意义。在本研究中，重组自交系 (RIL) 种群和全基因组关联研究 (GWAS) 面板分别包含 193 个品系和 379 个种质，用于 SMV 抗性的 QTL 定位。连锁作图鉴定了13 号染色体上的一个主要 QTL，qSMV13，赋予对 SMV SC3 和 SC7 菌株的抗性，分别解释了 71.21% 和 76.59% 的表型变异。QTL qSMV13靠近已知的 SMV 抗性基因座rsv1-h。GWAS分析揭示与电阻显著关联到SC3染色体2，11，13，14和16其中一个SNP标记，ss715614844五个单核苷酸多态性（SNP），为侧翼的标记物的权利qSMV13。结合连锁作图和 GWAS 分析使我们能够在包含七个基因的 97.2-kb 基因组区域中界定qSMV13。提出LRR-RLK蛋白作为qSMV13的候选基因。这些结果为大豆分子育种计划中的 SMV 抗性提供了选择标记和候选基因。