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Daily dynamic of transcripts abundance of Ma-LMM01-like cyanophages in two lowland European reservoirs
Ecohydrology & Hydrobiology ( IF 2.6 ) Pub Date : 2021-08-18 , DOI: 10.1016/j.ecohyd.2021.07.003
Aleksandra Jaskulska 1, 2 , Arnoldo Font Nájera 1, 2 , Piotr Czarny 3 , Liliana Serwecińska 2 , Joanna Mankiewicz-Boczek 1, 2
Affiliation  

The light-dark cycle seems to influence the infection pattern of cyanophages. This may be related to their dependence on the photosynthetic process of their hosts - cyanobacteria. The present study examined the daily occurrence dynamic of Microcystis with the use of the 16S rRNA gene and the Ma-LMM01-like cyanophages with the use of the g91 gene in Jeziorsko and Sulejow reservoirs (Poland). Also, the daily expression pattern of mentioned cyanophages’ gene was investigated. The daily dynamic of cyanophage was mainly associated with Microcystis occurrence. However, the g91 transcription exhibited greater activity during daylight and lowered within nighttime in both water bodies. The maximum transcription was found at 4:00 PM in Jeziorsko and 12:00 PM in Sulejow reservoirs. Additionally, the g91 gene fragments shared a high level of similarity with Microcystis specific cyanophages Ma-LMM01 and MaMV-DC strains.



中文翻译:

欧洲两个低地水库中 Ma-LMM01 样噬蓝体转录本丰度的每日动态

明暗循环似乎会影响噬蓝体的感染模式。这可能与它们对宿主——蓝藻的光合作用过程的依赖有关。本研究在 Jeziorsko 和 Sulejow 水库(波兰)中使用 16S rRNA 基因和使用g91基因的 Ma-LMM01 样噬蓝体检查了微囊藻的日常发生动态。此外,还研究了上述噬蓝体基因的日常表达模式。噬蓝体的日常动态主要与微囊藻的发生有关。然而,g91在两个水体中,转录在白天表现出更大的活性,而在夜间则降低。在 Jeziorsko 下午 4:00 和 Sulejow 水库下午 12:00 发现最大转录。此外,g91基因片段与微囊藻属特异性噬蓝细胞 Ma-LMM01 和 MaMV-DC 菌株具有高度相似性。

更新日期:2021-10-07
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