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Too Hot to Handle: Antibacterial Peptides Identified in Ghost Pepper
Journal of Natural Products ( IF 5.1 ) Pub Date : 2021-08-17 , DOI: 10.1021/acs.jnatprod.1c00281
Kevin D Culver 1 , Jessie L Allen 2 , Lindsey N Shaw 2 , Leslie M Hicks 1
Affiliation  

Capsicum spp. (hot peppers) demonstrate a range of interesting bioactive properties spanning anti-inflammatory, antioxidant, and antimicrobial activities. While several species within the genus are known to produce antimicrobial peptides (AMPs), AMP sequence mining of genomic data indicates this space remains largely unexplored. Herein, in silico AMP predictions were paired with peptidomics to identify novel AMPs from the interspecific hybrid ghost pepper (Capsicum chinense × frutescens). AMP prediction algorithms revealed 115 putative AMPs within the Capsicum chinense genome, of which 14 were identified in the aerial tissue peptidome. PepSAVI-MS, de novo sequencing, and complementary approaches were used to fully molecularly characterize two novel AMPs, CC-AMP1 and CC-AMP2, including elucidation of a pyroglutamic acid post-translational modification of CC-AMP1 and disulfide bond connectivity of both. Both CC-AMP1 and CC-AMP2 have little homology with known AMPs and exhibited low μM antimicrobial activity against Gram-negative bacteria, including Escherichia coli. These findings demonstrate the complementary nature of peptidomics, bioactivity-guided discovery, and bioinformatics-based investigations to characterize plant AMP profiles.

中文翻译:

太热了:鬼椒中发现抗菌肽

辣椒属 (辣椒)表现出一系列有趣的生物活性特性,包括抗炎、抗氧化和抗菌活性。虽然已知该属内的几个物种能够产生抗菌肽 (AMP),但基因组数据的 AMP 序列挖掘表明这一空间在很大程度上仍未被探索。在此,将计算机 AMP 预测与肽组学配对,从种间杂交鬼椒(Capsicum chinense × frutescens)中鉴定出新的 AMP。AMP 预测算法揭示了辣椒基因组内 115 个假定的 AMP ,其中 14 个是在空中组织肽组中鉴定的。使用 PepSAVI-MS、从头测序和互补方法对两种新型 AMP(CC-AMP1 和 CC-AMP2)进行全面分子表征,包括阐明 CC-AMP1 的焦谷氨酸翻译后修饰以及两者的二硫键连接性。CC-AMP1和CC-AMP2与已知的AMP几乎没有同源性,并且对革兰氏阴性菌(包括大肠杆菌)表现出低μM抗菌活性。这些发现证明了肽组学、生物活性引导的发现和基于生物信息学的研究的互补性,以表征植物 AMP 谱。
更新日期:2021-08-27
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