Glioblastoma (GBM), one of the most common primary intracranial malignant tumours, is very difficult to be completely excised by surgery due to its irregular shape. Here, we use an MRI/NIR fluorescence dual-modal imaging nanoprobe that includes superparamagnetic iron oxide nanoparticles (SPIONs) modified with indocyanine (Cy7) molecules and peptides (ANG or ^DANG) to locate malignant gliomas and guide accurate excision. Both peptides/Cy7-SPIONs probes displayed excellent tumour-homing properties and barrier penetrating abilities in vitro, and both could mediate precise aggregation of the nanoprobes at gliomas sites in in vivo magnetic resonance imaging (MRI) and ex vivo near-infrared (NIR) fluorescence imaging. However, compared with ANG/Cy7-SPIONs probes, ^DANG/Cy7-SPIONs probes exhibited better enhanced MR imaging effects. Combining all these features together, this MRI/NIR fluorescence imaging dual-modal nanoprobes modified with retro-enantio isomers of the peptide have the potential to accurately display GBMs preoperatively for precise imaging and intraoperatively for real-time imaging.

胶质母细胞瘤（GBM）是最常见的原发性颅内恶性肿瘤之一，由于其形状不规则，很难通过手术完全切除。^{在这里，我们使用 MRI/NIR 荧光双模式成像纳米探针，其中包括用吲哚菁 (Cy7) 分子和肽 (ANG 或D} ANG ) 修饰的超顺磁性氧化铁纳米粒子 (SPION)来定位恶性神经胶质瘤并指导准确切除。两种肽/Cy7-SPIONs 探针在体外均表现出优异的肿瘤归巢特性和屏障穿透能力，并且都可以在体内磁共振成像 (MRI) 和离体近红外 (NIR) 中介导纳米探针在神经胶质瘤部位的精确聚集荧光成像。然而，与 ANG/Cy7-SPIONs 探针相比，^DANG/Cy7-SPIONs 探针表现出更好的增强磁共振成像效果。将所有这些特征结合在一起，这种用肽的反对映异构体修饰的 MRI/NIR 荧光成像双模式纳米探针有可能在术前准确显示 GBM，以进行精确成像，并在术中进行实时成像。