The composition and location of charged and hydrophobic amino-acid residues of the collagenase molecule from Clostridium histolyticum were investigated. The percentage ratio of the various amino-acid types on the enzyme surface was determined. It was established that the charged and hydrophobic amino-acid residues are unevenly distributed over the surface of the molecule, forming local clusters. It was suggested that the most promising supports for immobilization of bacterial collagenase are hydrophobic or non-polar polymers that are likely to interact with a region on the surface of the enzyme molecules that is remote from the active center. It was established that gels of chitosan and chitosan succinate are more promising supports for immobilization of collagenase than the cation-exchange fiber VION KN-1 and the anion-exchange fiber VION AN-1.

溶组织梭菌胶原酶分子带电荷和疏水氨基酸残基的组成和位置被调查。测定酶表面上各种氨基酸类型的百分比。已确定带电荷和疏水性氨基酸残基不均匀分布在分子表面，形成局部簇。有人提出，最有希望固定细菌胶原酶的支持物是疏水性或非极性聚合物，它们可能与酶分子表面远离活性中心的区域相互作用。已经确定，与阳离子交换纤维 VION KN-1 和阴离子交换纤维 VION AN-1 相比，壳聚糖和壳聚糖琥珀酸盐凝胶是固定胶原酶的更有希望的载体。